中文摘要：

背景倡导者分析当前被使用在信号 transduction 和 transcriptional 规定的研究检测指向的基因的表示。作为记者基因，酶起一个重要作用并且在胚胎的肺成纤维细胞房间(2BS ) ， HeLa 房间和 MCF-7 房间是的倡导者 assay.Methods 人广泛地被使用了有各种各样的基因的 transfected 由 lipofectamine 嵌入。这研究决定了影响倡导者活动决心的各种各样的因素，例如记者基因和内部参考书的选择，剂量和带抄写因素的向量的类型，宿主细胞和 instruments.Results 酶试金的敏感比提高的绿荧光蛋白质( EGFP )的高得多。而且，倡导者活动仅仅在结果可以在外面被颠倒的某些范围以一种剂量相关的方式被增加，倡导者活动与正在带 cDNA.Otherwise 的表示向量有关，倡导者的长度，内部参考书和主人房间罐头也影响倡导者 activity.Conclusions 精确地检测倡导者活动，包括影响记者基因试金的剂量，向量，长度和主人房间的一些因素上述的 sho

英文摘要：

Background Promoter analysis is currently applied to detect the expression of the targeted gene in studies of signal transduction and transcriptional regulation. As a reporter gene, luciferase plays an important role and has been used widely in the promoter assay. Methods Human embryonic lung fibroblast cells （2BS）, HeLa cells and MCF-7 cells were transfected with various genes embedded by lipofectamine. This study determined vadous factors that affect promoter activity determination, such as the selection of the reporter genes and internal references, the dose and the type of the vectors carrying the transcription factors, the host cells and the instruments. Results The sensitivity of the luciferase assay was much higher than that of enhanced green fluorescence protein （EGFP）. Moreover, promoter activity is increased in a dose-related manner only in certain ranges outside of which the results may be reversed and the promoter activity is related to the expression vector which is carrying the cDNA. Otherwise, the length of the promoter, internal references and the host cell can also influence the promoter activity. Conclusions To detect the promoter activity accurately, a few factors including dose, vector, length and host cell which influence reporter gene assay aforementioned should be considered.