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E-选择素和巨噬细胞炎症蛋白2在慢性阻塞性肺疾病大鼠气道炎症中的作用

ISSN号：1671-6205
期刊名称：《中国呼吸与危重监护杂志》
时间：0
分类：R563[医药卫生—呼吸系统;医药卫生—临床医学;医药卫生—内科学] R587.1[医药卫生—内分泌;医药卫生—临床医学;医药卫生—内科学]
作者机构：[1]中南大学湘雅二医院呼吸内科,湖南长沙410011
相关基金：基金项目：湖南省卫生厅科研基金资助项目（编号：B2006-177）;教育部高等学校博士点专项科研基金资助项目（编号：20050533023）;湖南省2006年学位与研究生教育教学改革研究课题资助项目（编号：06807）

作者：罗曼[1], 陈平[1], 蔡珊[1], 张程[1], 邓建华[1], 刘绍坤[1], 陈剑波[1], 吴杰[1]

关键词：慢性阻塞性肺疾病, E-选择素, 巨噬细胞炎症蛋白2, Chronic obstructive pulmonary disease, E-selection, Macrophage inflammatoryprotein-2

中文摘要：

目的探讨E-选择素和巨噬细胞炎症蛋白2（MIP-2）在慢性阻塞性肺疾病（COPD）气道炎症中的作用。方法24只健康雄性SD大鼠随机分为COPD组和正常对照组，每组12只。COPD组大鼠采用单纯烟熏法制备COPD模型。采用免疫组织化学方法检测COPD组和对照组大鼠支气管肺组织中E-选择素的表达，ELISA方法检测血清、支气管肺泡灌洗液（BALF）、肺组织匀浆中MIP-2的含量。结果所建大鼠模型的病理及肺功能测定基本符合人类COPD的特点。COPD组大鼠模型中E-选择素在支气管肺组织血管内皮细胞的表达明显高于对照组（P〈0.05），与BALF中的中性粒细胞数呈正相关（r=0．809，P〈0．01）；MIP-2在BALF及肺组织匀浆含量显著高于对照组（P均〈0．05），且均与BALF中的中性粒细胞数呈正相关（r值分别为0．893和0．716，P值分别〈0．01和〈0．05）。结论E-选择素和MIP-2可能参与了COPD气道炎症过程。

英文摘要：

Objective To study the role of E-selection and macrophage inflammatory protein-2 （MIP-2） in the airway inflammation in a rat model of chronic obstructive pulmonary disease （COPD）. Methods Twenty-four male SD rats were randomly divided into a normal control group and a COPD group. The rat model of COPD was established by exposure to cigarette smoking. Lung function, pathologic features of lung tissues and inflammatory cell differentials in bronchoalveolar lavage fluid （ BALF ） were investigated. Immunohistochemistry was employed to examine the expression of E-selection in lung tissue. The levels of MIP-2 in BALF,serum and lung tissues were measured with ELISA. Results The changes in histopathology and pathophysiology in the rat COPD model were similar to those in COPD patients. The expression of E-selection on bronchopulmonary vein endothelial cells of the COPD group significantly increased as compared with the normal control group （ P 〈 0. 05 ） and was positively correlated with the neutrophil counts in BALF in the COPD group （ r = 0. 809, P 〈 0. 01 ）. The levels of MIP-2 in BALF and lung tissues in the rats with COPD were signieantly higher than those in normal rats, both of which were positively correlated with the neutrophil counts in BALF in the COPD group （ r = 0. 893, P 〈 0. 01 ; r = 0. 716,P 〈 0. 05）. Conclusion Both E-selection and MIP-2 may be involved in the process of airway inflammation in COPD.