中文摘要：

通过用葡萄糖培养天府肉鹅（A nser cygnoides）原代肝细胞的试验，探讨葡萄糖对肝细胞脂质合成能力的影响．研究发现：0～25mmol／L葡萄糖对肝细胞上清液中谷草转氨酶（aspartate transaminase, AST）、谷丙转氨酶（alanine amiotransferase, ALT）浓度没有显著影响，35mmol／L葡萄糖影响显著，但AST、ALT浓度均在正常范围内，表明肝细胞功能正常；油红氧染色实验结果表明：葡萄糖处理后显著增加细胞内脂滴集聚和脂质沉积；25、35mmol／L葡萄糖能显著增加乙酰辅酶A羧化酶α（acetyl-CoA carboxylasea, ACCα）、脂肪酸合成酶（fattyacid synthetase, FAS）酶活性；5、25、35mmol／L葡萄糖都能显著增加ACCα、FAS基因mRNA表达量．因此．葡萄糖能通过上调ACCα、FAS基因mRNA表达量及酶活性诱导肝细胞内脂质沉积．

英文摘要：

The goal of this experiment was to investigate the effects of glucose on lipids deposition in primary hepatocytes of Tianfu meat goose （Anser cygnoides）. The results indicated： 0-25 mmol/L glucose had no sig-nificant effect on the content of aspartate transaminase （AST） and alanine amiotransferase （ALT） in cell cul-ture supernatant. Although 35 mmol/L glucose had an obvious effect, the concentrations of AST and ALT within normal range. That＇s to say, hepatocytes is fine. Furthermore, the results of Oil Red-O staining revealed that the glucose significantly promoted lipid droplet accumulation and lipid deposition. Compared with the control group, lipids accumulation, acetyl-CoA carboxylase a significantly increased by 25 and 35 mmol/L glucose （ACCα） and fatty acid synthetase （FAS） activity were 5, 25, and 35 mmol/L glucose increased significantly the mRNA expression of A CCα and FAS. In conclusion, glucose could induce hepatic lipids deposition by up-regulating gene expression and enzyme activity of A CCα and FAS in goose hepatocytes.