中文摘要：

目的：探讨 Toll 样受体2（TLR2）及其信号通路下游因子在 HIV /结核分枝杆菌（M TB）双重感染者中的表达。方法选择 HIV 单纯感染者32例、HIV/M TB 双重感染者30例、M TB 单纯感染者28例，以及健康对照者29名作为观察对象。采集外周静脉血，检测血清 HIV 载量，实时定量 PCR （qPCR）检测 PBMC TLR2 mRNA 相对表达量，流式细胞术检测外周血单核细胞 TLR2平均荧光强度（MFI）表达水平，ELISA 法测定血清 IL-6、TNF-α。统计学处理采用卡方检验、t 检验、方差分析和秩和检验。结果 HIV /M TB 组的病毒载量为（5．113±1．018） lg 拷贝/mL ，HIV 组为（4．416±1．020） lg 拷贝/mL ，两组比较差异有统计学意义（t ＝3．449，P＜0．01）；HIV 组、HIV /M TB 组、M TB 组和健康对照组 PBMC TLR2 mRNA 相对表达量分别为1．397±0．601、1．463±0．702、1．429±0．630和0．970±0．488，4组间比较差异有统计学意义（ F ＝4．197，P ＝0．007）；4组单核细胞 TLR2的 MFI 分别为28．12±4．55、38．11±11．77、31．13±12．10和23．33±5．14，4组间比较差异有统计学意义（F＝13．976， P＜0．01）。4组血清 IL-6、TNF-α水平比较，差异有统计学意义（Z 值分别为19．088、15．475，均 P ＜0．01），3个病例组的 IL-6表达均比健康对照组高，而 TNF-α表达均比健康对照组低。结论 HIV /M TB 双重感染有可能加重 TLR2的活化，且促进 IL-6的表达上调。

英文摘要：

Objective To explore the expressions of Toll-like receptor 2 （TLR2 ） and the downstream proteins in patients with human immunodeficiency virus /Mycobacterium tuberculosis （HIV /M TB） co-infection .Methods A total of 119 subjects were randomly enrolled .The subjects were divided into four groups ：HIV group （n = 32） ,HIV /M TB group （n = 30） ,M TB group （n = 28） and healthy control group （n= 29） .Peripheral venous blood was collected and the HIV-1 viral load was determined by standard method .The expression levels of TLR2 mRNA in peripheral blood mononuclear cells （PBMC） were determined by real-time quantitative PCR （qPCR） and mean fluorescent intensity （MFI） of TLR2 protein was detected by flow cytometry .The plasma interleukin （IL）-6 and tumor necrosis factor （TNF）-α levels were measured with enzyme-linked immunosorbent assay kits .The data were statistically analyzed by chi-square test ,students t test ,analysis of variance and rank sum test when appropriate .Results The viral load in HIV /M TB group （[5 .113 ± 1 .018] lg copy/mL ） was significantly higher than that in HIV group （[4 .416 ± 1 .020] lg copy/mL ; t = 3 .449 , P〈 0 .01） .The TLR2 mRNA expressions in PBMC 〈br〉 among HIV ,HIV/M TB ,M TB and healthy control groups were 1 .397 ± 0 .601 ,1 .463 ± 0 .702 ,1 .429 ± 0 .630 ,and 0 .970 ± 0 .488 ,respectively ,which was significantly different among the 4 groups （F =4 .197 , P= 0 .007） .The MFI of TLR2 protein expressions on PBMC among HIV ,HIV /M TB ,M TB and healthy control groups were 28 .12 ± 4 .55 ,38 .11 ± 11 .77 ,31 .13 ± 12 .10 and 23 .33 ± 5 .14 ,respectively . The TLR2 protein expression levels were significantly different among 4 groups （F= 13 .976 ,P〈 0 .01） . The plasma IL-6 and TNF-α concentrations were significantly different among 4 groups （Z = 19 .088 , 15 .475 ,both P〈 0 .01） .The IL-6 concentrations in three patient groups were higher than that in healthy control group ,but the TNF-α concentrations were