中文摘要：

目的探讨荧光镜下界定绿色荧光裸小鼠脑结构部位的可行性及优势所在。方法取表达绿色荧光蛋白（GFP）的成年裸小鼠脑置于SLY脑切片模具中，切出1mm或0．9mm厚的脑片，再分别对其进行25μm厚的冰冻切片。荧光镜下观察切片的形态结构，结合《小鼠脑立体定位图谱》中小鼠脑解剖资料，在荧光镜下界定其解剖结构。观察结束后，全部切片行尼氏染色做对照。结果依据脑内不同结构间的荧光色差可对其进行辨认：细胞核、尼氏小体密集区及神经束分布区为弱荧光信号；细胞核、尼氏小体密集区周围结构，如嗅球的丛状层、小脑的分子层为强荧光信号。切片经尼氏染色后，体视显微镜下观察到的脑结构与荧光镜观察的结果基本一致。结论荧光镜下界定绿色荧光裸小鼠脑结构部位可作为一个实验手段应用于荧光示踪实验的解剖定位研究。

英文摘要：

Objective To explore the feasibility and advantage of fluoroscope in identification of brain structures in nude mice with green fluorescent protein （GFP） expression. Methods We laid the whole brain separated from 8-week adult nude mice with GFP expression into SLY mouse brain blocker to produce slices of 1 or 0.9 mm thickness; and then, 25μ-thickness frozen sections were cut. Fluoroscope was employed to observe the morphological structure to define their anatomic structures with reference to The Mouse Brain in Stereotaxic Coordinates compiled by Paxinos. After the observation, these frozen sections were performed Nissl staining for contrast. Results Different structures can be identified by their distinct fluorescence intensity： the dense areas of nuclei, Nissl bodies and nerve tract showed low fluorescence intensity; while the structures around the areas of nuclei and nerve tract, such as, the plexiform layer of olfactory bulb and the molecular layer of cerebella, showed high fluorescence intensity. The fluorescence intensity was attenuated obviously after Nissl staining; the visualized structural information observed under stereomicroscope was in accordance with that viewed by fluoroscope. Conclusion The identification of brain structure in nude mice with GFP by fluoroscope can serve as an experimental platform being applied in the anatomic structure positioning in fluorescence tracer experiments.