中文摘要：

选择一段与COL1A1基因相应序列同源、适合于在大肠杆菌E．coli中表达、含有编码促细胞粘附位点GER三肽密码子的基因序列。利用RT-PCR技术从人组织扩增该序列，构建pET28a（＋）-COL重组表达质粒并转化到大肠杆菌BL21（DE3）pLysS。IPTG诱导表达获得重组人源性胶原RHDC，表达量达到菌体总蛋白的40％左右，SDS—PAGE和Western blot结果推测该胶原具有三螺旋结构，细胞粘附试验证明RHDC具有促细胞粘附能力，有应用于生物材料领域的潜力。

英文摘要：

The DNA sequences, which originated from gene COL1A1, optimized for E. coli codon usage and contained cell adhesion domains of GER units, was amplified by RT-PCR technology from human tissue. The recombinant expression vector pET28a（ ＋ ）-COL encoded the recombinant human-derived collagen （RHDC） was constructed and transformed host E. coli strain 13/21 （DE3）pLysS. The RHDC production was induced by the addition of IPTG and approximately 40% of the total strain proteins. RHDC could form a triple-helical structure suggested by the SDS-PAGE and Western blot analysis. Cell adhesion assay indicated that RHDC had high cell adhesive character, which makes it suitable for use as biomaterials.