中文摘要：

目的：预测和分析人颗粒溶素（Granulysin,GNLY）蛋白的结构与功能.方法：采用生物信息学的方法对GNLY迸行系统分析与研究,包括GNLY的理化性质、跨膜区和引导肽、亚细胞定位、二级结构功能域、蛋白质的功能分类预测、多重序列比对与系统发育树构建、三级结构建模等.结果：GNLY蛋白分子量为15.33kD,等电点为9.35,具有引导肽（N端1-22个氨基酸序列）无跨膜区,该蛋白属于分泌性蛋白;其二级结构主要为α螺旋和无规则卷曲,包含1个SapB结构域,三级结构显示结果与二级结构预测结果相符.蛋白质多重序列比对和聚类分析显示,人GNLY蛋白与牛、猪及艾鼬等哺乳动物的GNLY蛋白具有一定的同源性.结论：GNLY生物信息学数据的探索,可为GNLY蛋白的功能研究提供参考资料和理论依据.

英文摘要：

Objective To study the structure and biological function of the human granulysin. Method： Many bioinformatics methodswere used to analysis physical and chemical properties, hydrophobicity, transmembrane region, signal peptide, secondary structure, functionaldomain and assortment, multiple alignment, phylogenetic tree and three-dimensional structures of GNLY. Result： The results showed that GNLYwas hydrophilic protein, whose calculated molecular mass was 15.33 kD, and theoretical isoelectric point was 9.35, with one signal peptide （the N-terminal 1-22 amino acid） and without transmembrane regions. The protein secondary structure were a-helix and random coil and contains a SapBmotif. The three -demensional structure prediction showed similar results with secondary structure prediction. The multiple alignment andclustering analysis showed that GNLY is an orthologous protein belong to saposin-like protein （SAPLIP）family, and has similarity to Bos taurus,Sus scrofa and Mustela putorius furo GNLY. Conclusion： The bioinformatics analysis will be helpful for the further study of the function of GNLY.