中文摘要：

角蛋白家族包含表皮软角蛋白和毛发硬角蛋白，可以分为酸性Ⅰ型和碱性或中性的Ⅱ型角蛋白亚家族。从构建的成年山羊皮肤cDNA文库和ESTs分析中发现了和与绵羊羊毛角蛋白8C1和人Ⅰ型毛发角蛋白相应cDNA序列高度同源的序列，经序列分析证明是一个山羊毛发特异性角蛋白，命名为山羊Ⅰ型毛角蛋白1（gHa1），Gen Bank序列号为AY510110．1。推导的读码框ORF由413氨基酸组成，与绵羊8C1角蛋白的序列一致性为97．8％。原位杂交显示它在皮肤初级和次级毛囊的皮质层有强烈的表达。

英文摘要：

The keratin famil be divided into acidic type y includes epithelial （soft） keratins and hair （hard） keratins, and can Ⅰ and basic to neutral type Ⅱ subfamilies. A eDNA was library constructed with poly （A）^＋ RNA from adult goat skin and 392 ESTs were analyzed. Based on sequence homology comparisons with the known type I hair keratins sheep 8C Ⅰ and hHal, A fulllength eDNA that encoded goat hair keratin gila Ⅰ （GenBank Accession No. AY510110. 1） could be identified, it derived 413 amino acid sequence comparisons with the sheep 8C1 type Ⅰ mRNA exhibits the highest homology （97.8%）. In site hybridization with the gila1 cRNA probe on skin revealed a strong expression in cortex of both primary and secondary hair follicles.