中文摘要：

自噬对肝再生有积极的作用，但具体作用机制仍有待阐明。为了解自噬在大鼠肝再生的变化和机理，通过蛋白质组学（iTRAQ方法）检测了大鼠肝再生中调控自噬的信号通路相关蛋白和自噬过程相关蛋白的变化。结果表明，调控自噬的P13K／Akt，mTOR，AMPK均被激活，泛素一蛋白酶体相关蛋白发生显著表达变化，溶酶体相关膜蛋白和水解酶发生显著变化。IPA分析发现，自噬在肝再生的启动阶段和进展阶段上调。根据研究结果，提出线粒体和溶酶体共存假说，并初步探讨并图示其存在的可能性和机理。

英文摘要：

elucidated. changes of process by Autophagy affects the liver regeneration （LR）. However, the detailed mechamsm remains to oe In order to explore the change and mechanism of autophagy in rat LR, we detected the expression proteins in the signal pathways which regulate autophagy and the proteins associated with autophagy isobaric tags for relative and absolute quantitation （iTRAQ） combined with mass spectrometry （MS）. The results indicated that the PI3K/Akt, mTOR and AMPK signaling pathways were activated. The ubiquitin- proteasome-related proteins and the membrane proteins and hydrolases associated with lysosome changed significantly. Autophagy up-regulated at the priming and progression stage in LR by the analysis from IPA. We proposed the hypothesis that lysosomes and mitochondria might coexist and explore the possible mechanism about that kind of coexistence.