以耐铝性较强的南京菊芋和耐铝性较弱的资阳菊芋为对比试验材料,在水培条件下,研究叶面喷施500μmol/L水杨酸(SA)对50μmol/L铝胁迫下菊芋幼苗叶片生长特性、光合特性、抗氧化酶活性及耐铝性的影响。结果表明,经过50μmol/L铝处理21d后,南京菊芋和资阳菊芋均受到一定的抑制,株高、根长及地上部分鲜重、叶绿素含量、最大光能转换效率(Fv/Fm)、最大荧光产量(Fm)及电子转移速率(ETR)显著降低,SOD、POD与CAT呈现相反的变化趋势,显示出2个菊芋品种的耐铝性差异,初始荧光(F)、可溶性蛋白含量、超氧阴离子产生速率(O2-)、游离脯氨酸含量(Pro)以及MDA含量均上升。喷施500μmol/L SA后显著提高菊芋的株高、根长及地上部分鲜重、叶绿素含量及叶绿素荧光相关指标、抗氧化酶活性;降低叶片的超氧阴离子产生速率(O2-),可溶性蛋白含量、游离脯氨酸含量(Pro)和丙二醛(MDA)含量呈现上升的变化趋势。通过灰色关联度分析可知,喷施SA对菊芋的叶绿素b、Fm和ETR影响最大;由隶属函数分析得出,每天喷施500μmol/L SA通过提高抗氧化酶活性和光合作用等途径缓解铝胁迫对菊芋造成的伤害,增强菊芋抵抗铝胁迫的能力。
A solution culture experiment was conducted to study the effects of exogenous salicylic acid(SA)(foliage spraying of 500μmol/L SA)on growth characteristics,photosynthetic characteristics,antioxidant enzyme activity and aluminum(Al)tolerance of 2Jerusalem artichoke(Helianthus tuberosus)cultivars,Nanjing(Al-resistant genotype)and Ziyang(Al-sensitive genotype)Jerusalem artichoke,under Al stress.The results showed that after growing under 50μmol/L Al stress for 21 days,seedings of both Helianthus tuberosus cultivars were negatively affected and their plant height,root length,fresh shoot weight,chlorophyll content,maximum light energy conversion efficiency(Fv/Fm),maximum fluorescence yield(Fm)and electron transport rate(ETR)significantly lowered.SOD,POD and CAT of 2 Helianthus tuberosus cultivars showed opposite trends,indicating the differences in Al tolerance of the 2cultivars,while initial fluorescence(Fo),soluble protein content,superoxide(O.2-)production rate,free proline content(Pro)and MDA of the 2cultivars of Helianthus tuberosus all increased.Spraying salicylic acid(SA)of 500μmol/L greatly improved the root length,fresh shoot weight,chlorophyll content and fluorescence indexes,and antioxidant enzyme activity,but reduced the O2-production rate.And the contents of soluble protein,free proline(Pro)and MDA also showed increasing trends.Through using grey correlation analysis,we found that spraying SA caused significant effects on chlorophyll b,Fm and ETR of Helianthus tuberosus.The results of subordinate function analysis indicated that by increasing antioxidant enzyme activity and photosynthesis,spraying 500μmol/L SA every day alleviated the damage to Helianthus tuberosus seedings caused by Al stress and enhanced the resistance of Helianthus tuberosus to Al stress.