中文摘要：

背景 Isocitrate lyase ( ICL )以前被表明 .Presently 在 Mycobacterium 肺结核(鱼雷快艇)的细胞内部的新陈代谢起一个枢轴的作用证据的 severallines 建议从鱼雷快艇( MTB-ICL )的 ICL 可以在在鱼雷快艇和主人 macrophage.However 之间的相互作用起一些作用，在 MTB-ICL 和主人 macrophage.Methods MTB-icl 和 M 之间的相互作用上没有研究。smegmatis (MS )-icl 基因被聚合酶链反应(PCR ) 放大并且克隆进 E。由 electroporation 获得 recombinant 梭 plasmids PMTB-icl 和 pMS-icl.Following 转变进 MS 的 coli-mycobacterium 梭 plasmid pUV15 ， pMTB-icl 和 pMS-icl 的表示被 recombinant plasmids 的 -PCR.The 表示从 pUVl 导出的反向的 transcriptase ( RT )验证 5 rMS 什么时候是由巨噬细胞的 phagocytized ，也经由荧光 microscope.Ms 1-2c 被验证， rMS-pUV15 ， rMS-pMS-icl 和 rMS-pMTB-icl 被用来感染 RAW264.7 房间，细胞内部的 MS 的 survivaI 是 mon

英文摘要：

Background Isocitrate lyase （ICL） was previously demonstrated to play a pivotal role in the intracellular metabolism of Mycobacterium tuberculosis （MTB）. Presently several lines of evidence suggest that ICL from MTB （MTB-ICL） may play some roles in the interaction between MTB and host macrophage. However, there has been no research on the interaction between MTB-ICL and host macrophage. Methods MTB-icl and M. smegmatis （MS）-icl genes were amplified by polymerase chain reaction （PCR） and cloned into the E. coli-mycobacterium shuttle plasmid pUV15 to obtain recombinant shuttle plasmids pMTB-icl and pMS-icl. Following transformation into MS by electroporation, the expression of pMTB-icl and pMS-icl was verified by reverse transcriptase （RT）-PCR. The expression of recombinant plasmids derived from pUV15 when rMS was phagocytized by macrophage was also verified via fluorescence microscope. Ms 1-2c, rMS-pUV15, rMS-pMS-icl and rMS-pMTB-icl were used to infect RAW264.7 cells and the survival of intracellular MS was monitored by bacterial culture at 0, 24 and 48 hours after infection. The culture supernatants from macrophage infected by Ms 1-2c, rMS-pUV15, rMS-pMS-icl and rMS-pMTB-icl were collected and the interferon （IFN）-y and nitric oxide （NO） concentrations were measured by ELISA or by Griess assay, respectively. The apoptosis of macrophage was assayed by the in situ TUNEL technique. Results RT-PCR showed that both pMTB-icl and pMS-icl could be expressed in MS. Fluorescence microscopic observation showed that recombinant plasmids derived from pUV15 （pUV15-iG） could also be expressed in MS when MS were phagocytized by macrophage. Bacterial culture data demonstrated that rMS-pMTB-icl exhibited significantly increased intracellular survival in the murine macrophage cell line RAW264.7 compared with Ms 1-2c, rMS-pUV15 and rMS-pMS-icl. This increased intracellular survival was not accompanied by the upregulation of IFN-y and NO in host macrophage. But a lower apoptosis rate of macropha