中文摘要：

目的研究柴胡Bupleuri Radix中多炔类化学成分及其在不同基原和不同产地柴胡中的量。方法采用硅胶柱色谱、反向色谱、Sephadex LH-20、制备色谱等色谱方法对柴胡中多炔类化学成分进行分离纯化,应用核磁等波谱技术鉴定化合物的结构;采用UPLC测定不同产地和不同基原的柴胡根中多炔类化合物的量。结果从柴胡95%乙醇提取物的石油醚部位中分离得到4个多炔类化合物,分别鉴定为（2Z,8Z,10E）-2,8,10-十五烷三烯-4,6-二炔-1-醇（1）、（2Z,8E,10E）-2,8,10-十五烷三烯-4,6-二炔-1-醇（2）、（2Z,8Z,10E）-2,8,10-十七烷三烯-4,6-二炔-1-醇（3）和柴胡炔醇（4）;经定量测定,结果表明不同基原和不同产地的柴胡中4种多炔类化合物的量差异较大,且产地差异大于种间差异。结论 4个化合物两两互为顺反异构体,且以异构体组合物存在时较稳定,其中,化合物1和3为新化合物,分别命名为柴胡炔醇A和柴胡炔醇C;UPLC测定法简便,稳定可靠,重复性好,灵敏度高,可作为柴胡中多炔类化合物的定量检测方法。

英文摘要：

Objective To study the separation of polyacetylenes and content of them in Bupleuri Radix from different bases and different habitats. Methods Silica gel, Sephadex LH-20 column chromatography, and preparative thin layer chromatography were used for the isolation and purification, the chemical structures were identified by NMR spectroscopic analyses, and the content of the polyacetylenes was determined by UPLC-PDA. Results Four polyacetylenes were isolated from the petroleum ether fraction of Bupleuri Radix and identified as（2Z,8Z,10E）-pentadecatriene-4,6-diyn-1-ol（1）,（2Z,8E,10E）-pentadecatriene-4,6-diyn-1-ol（2）,（2Z,8Z,10E）-heptadecatriene-4,6-diyn-1-ol（3）, and bupleurynol（4）. The contents of the polyacetylenes in Bupleuri Radix from various cultivation locations and origins were different, the locations may have greater impacts on the content than the species. Conclusion Compounds 1 and 3 are two new natural products named bupleurynol A and bupleurynol C, and the two compounds are more stable in the presence of Cis-trans isomers. The method is easy, accurate, reliable, and has the good reproducibility and high sensitivity, and can be used as a detection method for the polyacetylenes in Bupleuri Radix.