中文摘要：

目的探索艾灸治疗类风湿性关节炎（RA）抗炎的效应及预防骨破坏的IL-17信号转导机制。方法雄性SD大鼠66只,随机分为空白组、模型组、空白艾灸组、艾灸组、IL-17过表达模型艾灸组、IL-17沉默模型艾灸组、IL-17过表达模型组、IL-17沉默模型组,前6组每组各9只,后2组每组各6只。大鼠右后足足垫部皮内注入弗氏完全佐剂0.15mL制作RA模型。造模成功后第2天进行慢病毒注射建立IL-17基因过表达、IL-17基因沉默模型。RA造模后第7天开始麦粒灸大鼠肾俞、足三里2穴各5壮,每日1次,治疗18d。观测计算大鼠足跖肿胀度、热痛域;采用ELISA法检测大鼠血清IL-17、RANKL、TGF-β含量。结果造模后大鼠右后足容积显著高于空白组（P〈0.01）,热痛域值显著低于空白组（P〈0.01）;艾灸后大鼠右后足容积显著低于模型组（P〈0.01）,热痛域显著高于模型组（P〈0.01）。模型组大鼠血清IL-17、RANKL显著高于空白组,血清TGF-β含量显著低于空白组（P〈0.01）;艾灸后艾灸组大鼠血清IL-17、RANKL显著低于模型组,血清TGF-β含量显著高于模型组（P〈0.01）。IL-17过表达模型艾灸组大鼠RANKL含量显著高于艾灸组（P〈0.01）;IL-17过表达模型艾灸组大鼠TGF-β含量显著低于艾灸组（P〈0.01）。IL-17沉默艾灸组大鼠RANKL含量显著低于艾灸组和空白组（P〈0.01）。结论艾灸可能通过调控IL-17,从而调节RA大鼠血清骨破坏因子RANKL以及骨保护因子TGF-β含量,起到抑制RA滑膜炎症后期骨破坏、保护骨组织的作用。

英文摘要：

OBJECTIVE To explore the IL-17 signal transduction mechanism of anti-inflammatory effect of moxibustion on RA rats.METHODS 66 SD rats were randomly assigned to control,model,control＋moxa,moxa,IL-17overexpression＋moxa,IL-17knockdown＋moxa,IL-17 overexpression and IL-17 knockdown groups.Complete freund＇s adjuvant were injected into the right paw of SD rats to establish RA model,and siRNAs were injected for IL-17overexpression/knockdown models.After 7d,moxibustion treatments were performed for 18 d.RESULTS The swelling degree of RA rats and the serum level of IL-17 and RANKL were significantly higher than control group（P〈0.01）,and the PWTL of RA rats,serum level of TGF-βwere significantly lower than control group.After 18 dof moxibustion treatment,the swelling degree of RA rats and the serum level of IL-17,RANKL were significantly lower than control group（P〈0.01）,and the PWTL of RA rats and serum level of TGF-βwere significantly higher than control group.In addition,the serum level of RANKL in IL-17knockdown＋moxa rats were significantly higher than moxa group（P〈0.01）;the serum level of RANKL in IL-17knockdown＋moxa rats were significantly lower than moxa group and control group（P〈0.01）.CONCLUSION Moxibustion may adjust the serum level of RANKL and TGF-βby supressed the serum level of IL-17 therefore relieving RA in RA rats.