中文摘要：

通过实验优化了葡聚糖筛分介质和运行缓冲溶液的浓度，采用十二烷基硫酸钠（SDS）无胶筛分电泳分离体系（10％（w／v）葡聚糖，0．1％SDS，10％甘油，0．2mol／LTris-硼砂，pH8．3的缓冲液）在自制的玻璃微流控芯片上高效分离了BODIPY衍生的蛋白质分子量标准样品，连续6次电泳所得迁移时间的相对标准偏差均小于0．50％。以6种蛋白质分子量的对数对其迁移时间作图，线性回归良好（r=0．994）。采用该芯片电泳分析体系对免疫球蛋白G不同片段的分子量进行了测定，所得结果与实际基本相符。

英文摘要：

The concentrations of dextran sieving matrix and running buffer were optimized. Under the optimized conditions （ 10% （w/v） dextran, 0.1% SDS, 10% glycerol, 0.2 mol/L Tris-boric acid, pH 8.3）, six standard proteins could be efficiently separated using sodium dodecyl sulfate （SDS）-nongel sieving electrophoresis on home-made glass microfluidic chip. The relative standard deviations （RSDs） for migration time were lower than 0.50%. A good linear relationship （r =0.994） was built between the migration time of each protein and the logarithm of its molecular weight （logMr）. Using the chip-based electrophoretic separa- tion system, the molecular weights of different fragments of immunoglobulin G after different treatments were measured, and the results matched the facts.