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中文摘要：
研究了微珠阵列芯片传感单元的生物分子固定化方法．运用羰基二咪唑（CDI）法进行羧基微珠表面化学活化研究及表征．结果表明微珠表面咪唑基团的有效活化率为30．1％．活化微珠表面固定化抗体后，采用荧光显微成像分析，CDI法活化的微珠比未活化的微珠荧光强度提高60倍，活化效果显著．
英文摘要：
An approach of bio-molecular immobilization on the surface of the carboxyl microbeads, the sensing units of the microbead arrays, is presented. In this method, carboxyl microbeads were activated using N, N-Carbonyldiimidazole （CDI）, and the activated groups and the activation efficiency were quantitatively measured and calculated. As a result of the immobilization of the antibodies on the activated microbeads and the fluorescence microscopic imaging analysis, the fluorescence signal of the activated beads is 60-fold higher than that of the unactivated beads, which shows high activation efficiency.
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