中文摘要：

目的：检测基质金属蛋白酶-26（ MMP-26）在人胰腺癌中的表达并探讨其在胰腺癌进展中的作用及意义。方法通过免疫组化方法、逆转录聚合酶链反应（ RT-PCR）和蛋白质印记（ Western blot）分别检测MMP-26基因和蛋白在胰腺癌、癌旁正常组织中的表达，并探讨其与胰腺癌临床病理特征之间的关系。结果免疫组化显示MMP-26蛋白在胰腺癌中的阳性表达率（72%）比癌旁正常组织中（6%）明显升高，差异有统计学意义（ P〈0．05）；其高表达与胰腺癌的分化程度、TNM分期以及淋巴结转移有关（ P〈0．05）。 RT-PCR检测胰腺癌组织及癌旁正常组织各10例，有7例（70%）胰腺癌组织MMP-26 mRNA的表达高于癌旁正常组织。 Western blot法检测胰腺癌组织及癌旁正常组织各7例，发现有5例（71．4%）胰腺癌组织MMP-26蛋白的表达高于癌旁正常组织。结论 MMP-26的表达与胰腺癌的分化程度、TNM分期以及淋巴结转移均有相关性，有可能成为胰腺癌早期诊断的一个生物标志物及临床治疗的新靶点。

英文摘要：

Objective To detect MMP-26 expression in human pancreatic cancer and to investigate its role and significance in the progression of pancreatic cancer. Methods Immunohistochemistry, RT-PCR and Western blot were used to detect MMP-26 mRNA and protein in pancreatic cancer and adjacent normal tissue, to investigate the relationship between the clinical and pathological features of pancreatic cancer with MMP-26 . Results The positive expression rate of MMP-26 protein was significantly higher in pancreatic cancer （ 72%） than in adjacent normal tissues （6%） by immunohistochemical method, difference was considered statistically significant （P〈0. 05）. The high expression of MMP-26 was correlated with pancreatic differentiation, TNM stage and lymph node metastasis. RT-PCR detected 10 cases of pancreatic cancer tissues and adjacent normal tissues respectively, in which 7 cases （70%） were discovered the positive expression of MMP-26 mRNA was significantly unregulated in pancreatic tissue than in adjacent normal tissues. Western blot detected 7 cases of pancreatic cancer tissues and adjacent normal tissues respectively, in which 5 cases （71. 4%） were discovered the positive expression of MMP-26 mRNA was significantly higher in pancreatic tissue than in adjacent normal tissue. Conclusion The expression of MMP-26 is correlated with pancreatic differentiation, TNM stage and lymph node metastasis, may become a novel biomarker and clinical therapeutic target of pancreatic cancer.