中文摘要：

在转基因的动物的外长的基因的基因稳定性和表示效率是仔细，与集成有关，地点和拷贝数。在我们的实验室，由转基因的操作和随后的测试十字，我们建立了所有鱼生长荷尔蒙(GH ) 展出快生长的转基因的普通鲤鱼家庭。在这现在的研究，走的染色体，即时量的聚合酶链反应，和在 situ 杂交的荧光，技术被使用在转基因的普通鲤鱼识别外长的草鲤鱼 GH 基因的集成特征。外长的 GH 基因，在形式二完成并且一次不完全的双人脚踏车重复，在染色体对的结束附近被发现有综合进一个在富有的区域。我们假设外长的 GH 基因表示的高效率可能由于在染色体和在富有的集成地点的低拷贝数字。

英文摘要：

The genetic stability and expression efficiency of exogenous genes in transgenic animals are closely related to integration site and copy number. In our laboratory, by transgenic manipulation and subsequent test crosses, we established an ＂all-fish＂ growth hormone （GH） transgenic common carp family that exhibits fast growth. In this present study, genome walking, real-time quantita- tive polymerase chain reaction, and fluorescence in situ hybridization techniques were applied to identify the integration characteristics of the exogenous grass carp GH gene in the transgenic common carp. The exogenous GH genes, in the form of two complete and one incomplete tandem repeats, were found to have integrated into an AT- rich region near the end of a chromosome pair. We hypothesize that the high efficiency of exogenous GH gene expression might be due to the low copy number in the genome and the AT-rich integration site.