中文摘要：

目的初步探索衰老标记蛋白30（SMP-30）在人肺组织中的表达以及在慢性阻塞性肺疾病（慢阻肺）发病中的意义。方法纳入2012年1月至6月在江苏省人民医院因肺部肿瘤行肺叶切除术的患者20例。肺组织标本从江苏省人民医院组织库申请获得,为距离癌组织〉5 cm的相对正常组织。根据患者肺功能及吸烟情况,将患者分为健康对照组7例,吸烟对照组7例,以及慢阻肺组6例。采用免疫组织化学和蛋白免疫印迹法检测SMP-30在各组肺组织中的细胞定位及含量。结果 SMP-30在人肺组织中主要分布于肺泡巨噬细胞胞浆,慢阻肺组肺泡巨噬细胞及SMP-30阳性细胞数量均明显增多。蛋白免疫印迹法显示肺组织SMP-30蛋白在吸烟非慢阻肺组的含量明显高于健康对照组（2.16±0.23比1.10±0.14,P〈0.01）。相较于吸烟非慢阻肺组,慢阻肺组SMP-30蛋白表达进一步增加（4.62±0.97比2.16±0.23,P〈0.05）。结论 SMP-30的表达增加可能与肺泡巨噬细胞寿命延长、数量增多有关,有可能是慢阻肺发生发展的一个病理环节。

英文摘要：

Objective To explore the expression of senescence marker protein-30（ SMP-30） in human lung tissues and the significance in the pathogenesis of chronic obstructive pulmonary disease（ COPD）. Methods Lung tissue specimens（ 5 cm away from cancerous tissues） obtained by surgery resection in 20 subjects with solitary peripheral carcinoma in Jiangsu Province Hospital were investagted. The subjects were divided into three groups according to lung function and smoking history,ie. a COPD group（ 6 cases）,a healthy smoking group（ 7 cases） and a healthy control group（ 7 cases）. Immunohistochemistry and Western blot were used to determine the distribution and expression of SMP-30 in human lung tissues. Results SMP-30 protein mainly expressed in the cytoplasm of alveolar macrophages（ AM）.The numbers of AM and SMP-30-positive AM were significantly increased in the COPD group. Western blot analysis confirmed a significant increase in SMP-30 expression in the healthy smokers compared with the non-smokers（ 2. 16 ± 0. 23 vs. 1. 10 ± 0. 14,P 0. 01） and further enhanced in the patients with COPD compared with the healthy smoking subjects（ 4. 62 ± 0. 97 vs. 2. 16 ± 0. 23,P 0. 05）. The levels of the protein in different groups were： COPD group smoking group control group with significant difference.Conclusion These results suggest that SMP-30 expression may be involved in the mechanism of prolonged survival and the increase in number of AM and may be involved in the pathogenesis of COPD.