中文摘要：

目的：分析不同临产状态子宫下段平滑肌组织中与NF-κB相互作用的差异蛋白质,为分娩机制的研究提供新的实验及理论依据.方法：分别采集足月未临产、自然临产和前列腺素E2栓诱导临产（药物临产组）的孕妇子宫下段平滑肌组织.Western印迹验证NF-κB P65表达;联合免疫共沉淀、SDS-PAGE、电喷雾串联质谱（LC-ESI-MS/MS）和蛋白质生物信息分析等技术,分析不同临产状态子宫平滑肌组织中与NF-1cB相互作用的差异蛋白质,并利用Western印迹进行验证.结果：人类临产前后子宫下段平滑肌组织中NF-κB表达均为阳性,与NF-κB P65相互作用的差异蛋白,自然临产前后鉴定出9个,药物临产前后鉴定出5个,其中不同临产状态共同差异表达的蛋白3个,分别为热休克蛋白70（heat shock 70 kD protein,HSP70）、膜联蛋白A6和结蛋白,经Western印迹验证与蛋白表达相符.这些差异蛋白分别属于分子伴侣、信号转导、细胞骨架和能量代谢相关的蛋白质.结论：子宫平滑肌细胞中NF-κB通过与分子伴侣、信号传导、细胞骨架和能量代谢等相关的多种蛋白质相互作用,参与分娩的启动或调节.

英文摘要：

Objective： To analyze the differentially expressed proteins which interacted with NF-kappaB in the uterine lower segment smooth muscle tissues under different status of labor onset, and to provide a new foundation on the mechanisms for labor onset.Methods： NF-κB P65 protein expression in smooth muscle tissues from the term non-labor group, natural term labor group and drug-induced term labor group was analyzed by Western blot. Co-immunoprecipitation and SDS-PAGE （sodium dodecyl sulfate polyacrylamide gel electrophoresis） were performed to detect the proteins interacting with NF-κB p65 in the NF-κB p65 complexes. The components of the complex were identified by LC-ESI-MS/MS （liquid chromatography- tandem electrospray mass spectrometry） and database analysis. The identified differentially expressed proteins were confirmed by Western blot.Results： Positive expression of NF-κB was detected in all of the three groups. 10 differentially expressed proteins were identified by LC-ESI-MS/MS in human lower segment myometrium tissues in the term non-labor group and natural term labor group, mean while, 5 differentially expressed proteins were identified in the term non-labor group and the drug-induced labor group. 3 differential expression proteins were detected in all of the 3 groups, including Heat shock 70, Annexin A6 and Desmin, which were verified by Western blot. These proteins were mainly involved in chaperone, signal transduction, cell structure, and enertrv metabolism process,respectively.Conclusion： NF-κB expressed in uterine smooth muscle cells is involved in the process of initiation and regulation of labor onset through a number of proteins relevant to signal transduction, cell structure and energy metabolism.