中文摘要：

采用稀释平板法分离马铃薯瓢虫肠道菌,利用刚果红平板法对产纤维素酶菌株进行初筛,选取透明圈较大的菌株进行摇瓶发酵复筛,根据菌株形态、生理生化特征对菌株进行初步鉴定,通过正交法优化产酶条件。结果表明,经初筛和复筛得到1株酶活相对较高的菌株B-12,经初步鉴定为芽孢杆菌属（Bacillus sp.）。1.25%麦芽浸粉为碳源、1.5%KNO3为氮源、0.2%的NaCl、0.1%的CMC-Na、接种量6%、培养时间44 h为B-12产酶的适宜条件。优化后发酵液中的内切葡聚糖酶活（CMCA）为111.710 U/mL,较培养44 h后的酶活提高了8.78%;滤纸酶活（FPA）为35.017 U/mL,提高了387.23%;β-葡萄糖苷酶酶活（BGL）为116.799 U/mL,提高了700.38%。

英文摘要：

The bacteria from the intestine of Henosepilachna vigintioctomaculata were isolated through the pour plate method.They were preliminarily screened using Congo red medium plate method,and then screened according to their cellulase activities by flask shaking fermentation method.The strain was identified based on morphological and physiological characters.In addition,the culture substrates and fermentation conditions were optimized by orthogonal experiment method.A high cellulase-producing strain B-12 was screened from the intestine of H.vigintioctomaculata and it was identified as Bacillus sp..The best culture conditions were as follows： substrate were 1.25% malt meal,1.5% KNO3,0.2% NaCl,and 0.1% CMC-Na,inoculation quantity was 6%,culture time was 44 h.In this condition,the enzyme activity of CMCase,filter paper enzyme（FPA）,and β-glucosidase（BGL） were 111.710,35.017,and 116.799 U/mL respectively,which were 8.78%,387.23% and 700.38% higher than those of the initial strain respectively.