多巴胺是脑内一种天然的神经递质,对于体外培养的SH—SY5Y细胞,不同浓度的多巴胺会对其产生不同的影响。本文研究了一系列浓度梯度的多巴胺对SH—SY5Y细胞生长状态的影响,得出了其从对细胞生长促进作用转入毒性作用的拐点。并且对这些细胞中的多巴胺转运蛋白进行了染色,观察了它们在不同多巴胺浓度下,在细胞膜表面及细胞内部的分布规律。
Dopamine is a natural neurotransmitter in the brain. To the SH-SY5Y cells, different concentrations of dopamine will cause different effects. We investigated the effect of a series of dopamine concentration to SH-SY5Y cells, and got the inflexion between the acceleration and the toxicity. And we also observed the change of the dopamine transporter's distribution by the immunofluorescence with the different concentration of dopamine.