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野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶(PAL)活性的变化
  • ISSN号:1000-9841
  • 期刊名称:《大豆科学》
  • 时间:0
  • 分类:S565.1[农业科学—作物学]
  • 作者机构:[1]黑龙江省农业科学院博士后工作站,黑龙江哈尔滨150086, [2]东北林业大学博士后流动站,黑龙江哈尔滨150040, [3]东北农业大学大豆研究所,国家教育部大豆生物学重点实验室,黑龙江哈尔演150030, [4]黑龙江省农业科学院绥化分院,黑龙江绥化152052, [5]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086, [6]中国农业科学院作物科学研究所,北京100081
  • 相关基金:黑龙江省农业科学院博士后基金资助项目(LRB06-010);中国博士后基金资助项目(20060400835);国家自然科学基金资助项目(30671317,30810103063,30400285);黑龙江省自然科学基金资助项目(C200814);黑龙江省新世纪人才培养计划资助项目(NCET-06-007);博士后落户黑龙江科研启动基金资助项目(LBH-Q05032);国家重点基础研究发展计划资助项目(2004CB117203-4).
中文摘要:

对大豆疫霉根腐病菌胁迫下抗感不同野生大豆品种根、茎、叶中苯丙氨酸解氨酶(PAL)活性的变化规律进行了研究。结果表明:在接种疫霉根腐病菌1号生理小种后,抗病野生大豆根和茎中的PAL活性在病程的大部分阶段比相应对照增加,并且变化的幅度较大,而感病品种相反。抗感野生大豆叶中PAL活性与对照相比变化幅度均较小。

英文摘要:

Phytophthora root and stem rot caused by Phytophthora sojae is a destructive disease in soybean production regions all around the world. Some protective enzymes begin to be active when soybean is infected by P. sojae. Of the enzymes, phenylalanine ammonia-lyase(PAL) is a key and rate-limiting enzyme in phenyl propane metabolize pathway which could produce kinds of antimicrobial products. There is abundant wild soybean in China, but there are few reports on the relationship about PAL and resistance of wild soybean to P. sojae. In this paper,4 resistant wild soybeans and 4 susceptible wild soybeans were inoculated with zoospores of P. sojae race 1 as treatment and those inoculated with water as control. The PAL activity in roots, stems, and leaves were measured after inoculation of 0,12,24,36,48,60, and 72 h in the treatment and control respectively. The results indicated that the PAL activity in roots and stems of resistant wild soybeans was higher than that of the control at most of the stages of pathogenctic process,but was lower than that of control for susceptible wild soybean. The change range of PAL activity in leaves of both susceptible and resistant wild soybeans were relatively low compared with that of control.

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期刊信息
  • 《大豆科学》
  • 北大核心期刊(2011版)
  • 主管单位:黑龙江农业科学院
  • 主办单位:黑龙江省农业科学院编辑出版中心
  • 主编:刘忠堂
  • 地址:哈尔滨市南岗区学府路368号
  • 邮编:150086
  • 邮箱:dadoukx@sina.com
  • 电话:0451-86668735
  • 国际标准刊号:ISSN:1000-9841
  • 国内统一刊号:ISSN:23-1227/S
  • 邮发代号:14-95
  • 获奖情况:
  • 1988年获黑龙江优秀期刊奖,1996年获黑龙江省科技情报二等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:12334