中文摘要：

目的 ：探讨白介素-11（interleukin-11,IL-11）在小鼠肝脏缺血再灌注中的作用。方法：将20只健康雄性C57BL/6小鼠随机分成正常对照组、假手术组、缺血再灌注组和IL-11处理组,各组5只。采用70%肝脏缺血再灌注模型（缺血1 h,再灌注6 h）。缺血再灌注组及IL-11处理组分别于术前2 h给予PBS及IL-11尾静脉注射。通过酶联免疫吸附试验（ELISA法）分别测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、白介素-6（IL-6）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平。通过光镜观察组织学苏木精-伊红（HE）染色改变。应用反转录酶-聚合酶链反应（RT-PCR）检测肝组织IL-6、IL-1β、TNF-α水平。结果 ：缺血再灌注组及IL-11处理组肝酶水平明显高于正常对照组及假手术组（P〈0.05）。同时IL-11处理组又明显低于缺血再灌注组（P〈0.01）。缺血再灌注组镜下可见大面积肝细胞水肿及少量嗜酸性变,而IL-11处理组肝细胞水肿明显少于缺血再灌注组。IL-11处理组血清及肝组织中IL-6、IL-1β、TNF-α表达水平明显低于缺血再灌注组（P〈0.05）。结论：IL-11预处理可以通抑制IL-6、IL-1β、TNF-α的表达来减轻小鼠肝脏的缺血再灌注损伤。

英文摘要：

Objective：To investigate the effects of IL-11 on mouse liver warm ischemia/reperfusion （WI/Rp） injury. Methods： A total of 20 healthy male C57BL/6 mice, weighing （22 ± 3） g, were randomly divided into four main experimental groups （n=5 each）, including normal group, sham group, ischemia/repeffusion（I/R） group, and IL-11 pretreatment group. We chose a nonfatal model of 70% liver WI/Rp （treated with 1 h ischemia ,and then 6 h repeffusion）. The mice of I/R group were injected with PBS, and IL-11 pretreatment group with IL-11, at 2 h before operation. The levels of alanine aminotransferase（ALT）, aspartate aminotransferase （AST）, interleukin-6 （IL-6）, interleukin-1β （IL-1β） and tumor necrosis factor-alpha （TNF-α） in serum were detected by enzyme-linked immunosorbent assay（ELISA）. The mRNA expression of IL-6, IL-1 β and TNFα were examined by reverse transcription-polymerase chain reaction（RT-PCR）. Histological haema （HE） stained sections were histopathologically examined using light microscopy. Results： The liver enzyme levels were significantly increased in the I/R and IL-11 pretreatment group, compared to those in the normal and sham group （P 〈 0.05）. Meanwhile, the transaminase levels in the IL-11 pretreatment group were significantly reduced compared to those in the I/R group （ALT：P=0.003,and AST：P 〈 0.001, respectively）. Liver cell hydropic and acidophilic degeneration were increased in the I/R and IL-11 pretreatment group, compared to those in the normal and sham group. Besides, the cell hydropic and acidophilic degeneration in the IL-11 pretreatment group was significantly reduced, compared to that in the I/R group. The expressions of IL-6, IL-1β and TNF-α of blood serum and liver tissue were reduced in the IL-11 pretreatment group, compared to those in the I/R group （P=0.0025, P=0.0159, P=0.0175, P=0.0076, P=0.0005, and P=0.0004, respectively）. Conclusions： Pretreatment with IL-11 protects mouse livers from WI/Rp injury by s