利用触角电位和嗅觉测定技术,比较分析松墨天牛Monochamus alternatus Hope对马尾松(Pinusmassoniana Lamb)健康和松材线虫危害的枝条挥发物的触角电位和行为反应特点。实验结果表明:未交配松墨天牛对健康枝条挥发物的EAG反应值大于被害枝条的,交配后天牛对被害枝条的EAG反应值明显大于健康枝条的。在“Y”型嗅觉仪中,未交配天牛对健康枝条挥发物表现为正趋性,对被害枝条挥发物表现为负趋性,而交配后天牛对被害枝条挥发物表现为正趋性,对健康枝条挥发物表现为负趋性。说明不同发育时期的松墨天牛成虫对不同生理状态的寄主具有不同的敏感性和选择性。
Electroantennogram (EAG) and behavioural responses of Monochamus alternatus Hope at different developmental stages to extracts from healthy and pinewood nematode infested Pinus massoniana were investigated. The results indicated that the EAG response potential of unmated beetles to extracts of healthy branch was greater than that to extracts of infested branch,while reverse EAG resporse was observed in mated beetles had reverse result. “Y” tube olfactory tests revealed that unmated beetle had positive response to extracts of healthy branch and negative response to extracts of infested branch, whilst mated beetle, however, showed positive response to extracts of infested branch and negative response to extracts of healthy branch. The present study suggested that M. alternatus at developmental stages have special sensitivity and selectivity to host tree with different physiological conditions.