中文摘要：

通过PCR技术获得杜氏利什曼原虫中国山丘疫区分离株磷脂多糖1（LPG1）基因上游序列约2.2 kb,对该序列测序,根据测得序列设计引物,以染色体步移技术获得该序列下游约2 kb的核苷酸序列。将上述所获的2个序列拼接获得杜氏利什曼原虫中国山丘疫区分离株LPG1基因及其两侧非编码区核苷酸序列4 121 bp（GenBank登录号为HMO27899）。该序列与GenBank中相关序列进行序列一致性分析,结果显示,LPG1基因与多种利什曼原虫LPG1基因的核苷酸序列一致性为72%～78%。

英文摘要：

A fragment about 2.2 kb located at upstream of lipophosphoglycan 1 （LPG1）gene of Leishmania donovani isolate from hilly foci of China was obtained by PCR.The nucleotide sequence of the fragment was determined by sequencing.The sequence of the LPG1 gene and its downstream fragment （about 2 kb）was determined by using genome walking method.The above two fragment splicing result showed that the nucleotide sequence of the LPG1 gene with the noncoding region was 4 121 bp （GenBank accession number：HMO27899）.The BLAST results showed that the LPG1 geue of L.donovani isolate from hilly foci of China had 72%-78% nucleotide identity compared to that of other Leishmania spp. in GenBank.