从广陆矮4号/日本晴染色体片段代换系群体中筛选到1个株系C23011。该株系除第8染色体短臂上的一个片段来自广陆矮4号外,背景均与日本晴相同。陔株系在一然长日照条件下比轮回亲本日本晴提早抽穗约14.1d,但在短口照条件下,两者抽穗期无显著差异。利用BC1F2世代中筛选到的一个目标区段杂合但背景基本纯合的单株自交一代后得到的BC1F3分离群体进行遗传分析。在该群体中译株抽穗期呈不连续的舣峰分布,迟抽穗和早抽穗尊株比例符合3:1的分离比,表叫抽穗期受1个主效QTLqHd8.1控制。利用BC1F3群体中共1628株早抽穗的啦株将qHd8.1精细定化红BAC克隆AP005164上标记S8111和S849之间,物理距离约为59.94kh,该区间共有7个预测基因。
C23011 , containing a single introgressed segment of chromosome 8, was derived from the CSSL population with Nipponbare as the donor and Guanglu'ai 4 as the recurrent parent. In normal long-day conditions, its heading date was 14. I d shorter than that of Nipponbarc. Under short-day conditions, however, no obvious difference in betiding date between C27,011 and Nipponhare was observed. A segregation population derived from a BC, F2, individu,il with the heterozygous target segment was used for genetic analysis and gene mapping. The ratio of late heading plants to early heading plants showed a 3 : 1 frequency distribution, suggesting that the heading date wtriation was controlled by a single major QTL, which was named qHd8. 1. A physical map was constructed encompassing the qHdS. 1 locus and the locus was delimited to a 59.94 kb interval between markers S8111 and S849 on BAC clone AP005164 using 1628 individuals with shorter heading dale originated from the BC, F2, populations. There are seven predicted genes within this region.