中文摘要：

探讨鼠伤寒沙门菌在感染鼠巨噬细胞早期与细胞器的相互作用。用pTassC-GFP质粒转染鼠巨噬细胞RAW264.7,结合多抗的溶酶体标志物溶酶体相关膜蛋白-1用键合了Alexa594的羊抗鼠二抗显色,以观察标记了绿色荧光蛋白的TassC与溶酶体的关系;用pTassC-GFP和pDsRed2-Perxi质粒共转染RAW264.7细胞,以观察TassC-GFP与过氧化物酶体的关系;用SYTO42标记鼠伤寒沙门菌,感染用pTassC-GFP和pDsRed2-Perxi质粒共转染的RAW264.7细胞,以观察细菌与TassC和过氧化物酶体的关系。免疫荧光显示TassC-GFP不与鼠巨噬细胞RAW264.7中的溶酶体结合,但与标记了红色荧光的过氧化物酶体共定位;感染1 h的RAW264.7胞内SYTO42标记的鼠伤寒沙门菌吞噬泡可招募TassC-GFP和过氧化物酶体。这些发现提示在鼠伤寒沙门菌感染早期过氧化物酶体携带杀菌成分通过TassC介导可参与发挥一定的杀菌作用。

英文摘要：

Early interaction between Salmonella typhimurium（St） and organelle during the bacteria infection of mouse macrophages was investigated.Mouse macrophages RAW264.7 were transfected with pTassC-GFP plasmids to observe the relation of green fluorescence protein（TassC-GFP） vesicular structures and lysosomes.Lysosomal marker lysosome-associated membrane proteins-1 was detected with polyclonal antibodies and visulization with a goat-anti human IgG conjugated to Alexa 594.The interaction between TassC-GFP and peroxisomes were analyzed by co-transfection of pTassC-GFP and pDsRed2-Perxi plasmids to macrophages RAW264.7.St labeled SYTO42 was used to infect pTassC-GFP and pDsRed2-Perxi co-transfected macrophages RAW264.7 to observe relation among bacteria,TassC-GFP and peroxisomes.The results showed immunofluorescence indicated TassC-GFP did not bind with lysosomes,but co-localize with peroxisomes tagged with red fluorescence pDsRed2-Perxi in macrophages RAW264.7.And the infection for 1 h St-containing phagocytic vacuoles in RAW 264.7 cells labeled with SYTO42 could recruit or overlap TassC-GFP and peroxisomes.Therefore,these findings suggested that through TassC-mediated guidance the peroxisomes could play germicidal role in the early phase of St infection.