中文摘要：

目的探讨河北地区人群骨桥蛋白基因启动子-443T/C-、156delG/G和-66T/G三个多态性位点与冠心病发生之间的相关性。方法随机选择中国河北地区95例经冠状动脉造影检查并确诊为冠心病的患者及129例正常对照。提取外周血基因组DNA,采取特异性引物对所选区域进行PCR扩增,将扩增产物进行测序后统计基因型和等位基因在病例组和对照组中的分布情况。结果 -443T/C-、156delG/G两个SNP位点在冠心病患者组与正常人对照组中分布无明显相关性（P〉0.05）,而-66T/G位点在该地区不具备明显多态性。结论骨桥蛋白基因启动子区-443T/C、-156delG/G两个SNP位点与冠心病发病无关。

英文摘要：

Objective To investigate the relationship between coronary heart disease（CHD） and three single nucleotide polymorphism sites（-443T/C,-156delG/G,-66T/G） in the gene of osteopontin promoter region.Methods 95 CHD patients and 129 healthy volunteers were randomly selected from hospitals in Hebei province.DNA was extracted from peripheral blood samples.The target segment was amplified with specific primers.DNA sequencing was performed to detect the alleles.The distribution of genotypes and alleles in the two groups was analyzed.Results The distribution of genotypes and alleles for-443T/C and-156delG/G was not related to CHD（P〈0.05）.No detectable polymorphism for-66T/C was detected in this province.Conclusions The-443T/C,-156delG/G in the gene of osteopontin promoter might not be related to the CHD in Hebei province.