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中文摘要：
采用高灵敏度、信号稳定的化学发光分析法对血清中游离的人绒毛膜促性腺激素β亚单位 （Free hCGβ）进行检测, 并对免疫分析进行条件优化和改进, 建立化学发光磁酶免疫检测方法. 利用3-（2′-螺旋金刚烷）-4-甲氧基-4-（3″-邻氧酰苯基）-1, 2-二氧杂环丁烷（AMPPD）-碱性磷酸酶（ALP）化学发光体系, 用磁珠酶联免疫法对Free hCGβ进行检测, 其检测灵敏度较光度法提高了10倍, 线性范围为0.45～185.2 mIU/mL, 批间和批内相对标准偏差（RSD）均小于12%, 回收率为84%～120%之间, 临床标本的检测值与分光光度法的相关系数为0.955. 因此, 化学发光磁酶免疫分析法较分光光度法的精密度和灵敏度高, 可以作为检测血清中的Free hCGβ水平的一种可靠方法.
英文摘要：
In this paper, chemiluminescent assay with high sensitivity was used to detect free human chorionic gonadotrophin β subunit （Free hCGβ） in serum, which was based on antibody sandwich immunoassay separated by magenetie mierosphere in the aid of a planar magnetic field.. Alkaline phosphatase （ALP） was utilized as a labeled reagent of anti- Free hCGβ monoclonal antibody, which reacted with chemiluminescent reagent, 3（2′spiroadamantane）4-methoxy- 4（3″-phosphoryloxy） phenyl-1,2-dioxetane （AMPPD） in buffer solution and emitted photons. The sensitivity of the chemiluminescent method was 0.22 mIU/mL. The linear range was 0.45 - 185.2 mIU/mL. The intra- and extra- assay RSD values were below 8% and 15% . The recovery was in the range of 84% - 120%. The correlation coefficient was 0.955 in comparison with spectrophotometry immunoassay. This method can be practically applied to detect the concentration of free hCGβ in the clinical serum samples.
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