翻译控制肿瘤蛋白(translationally controlled tumor protein,TCTP)最初在鼠肿瘤细胞中被发现,研究表明TCTP广泛存在于动植物细胞中,并具有多种生物学功能。本研究用RT-PCR和RACE技术在抗白粉病栽培小麦Brock中克隆了一个TCTP基因,该基因全长766bp,推测编码一个168个氨基酸的多肽。ScanProsite分析表明,该多肽链具有2个TCTP特征结构区(TCTP1和TCTP2)和7个可能的功能位点。表达半定量分析发现,该基因受华北地区流行的优势小种15号白粉菌诱导,且随着诱导时间的增加其表达量增加。本研究将可能在小麦白粉病抗性研究领域开辟新的研究思路。
The translationally controlled tumor protein (TCTP) was initially designated as a find in mouse tumor cells. It is abundant in plant and animal cells and has many different functions. In this study,a TCTP gene was isolated as an Erysiphe graminis inducible cDNA cloned from the leaves of cultivar wheat Brock infected by E.graminis with RT-PCR and RACE technique. It encoded a polypeptide of 168 amino acids. After running a ScanProsite research,two specific TCTP signatures,named TCTP1 and TCTP2 and 7 conservation function sites were found. The studies on the expression of WbTCTP after infected by powdery mildew of race No.15 were carried out using sei-quantitative PCR technique. The expression level of WbTCTP gradually increased during the E.graminis infection. The results would provide a new way for the area of studying wheat powdery mildew resistance.