中文摘要：

采用RT-PCR结合RACE法,分离克隆龙眼（Dimocarpus longan Lour.）胚性愈伤组织生长素受体基因TIR1（Transport inhibitor response 1,即DL-TIR1）和生长素结合蛋白基因ABP1（Auxin biding protein 1,即DL-ABP1）,运用生物信息学方法对序列进行分析,并通过实时荧光定量PCR法研究其在龙眼体细胞胚胎（以下简称龙眼体胚）发生过程中的表达。结果表明：DL-TIR1为2328bp的mRNA全长序列（GenBank,GQ870264）,包含1个长为1761bp的开放阅读框,5′非编码区为118bp,3′非编码区为449bp,推定的氨基酸序列含586个氨基酸,与其它植物TIR1具有85%～57%同源性;DL-TIR1在龙眼体胚的各阶段均有表达,整个变化趋势呈近似＂W＂状,在子叶形胚中的表达量最高。DL-ABP1为869bp的mRNA全长序列（GenBank,GQ900592）,包含1个长为567bp的开放阅读框,5′非编码区为43bp,3′非编码区为259bp;推定的氨基酸序列含188个氨基酸,与其它植物ABP1具有87%～72%同源性,DL-ABP1在龙眼体胚中整个表达变化趋势也呈现近似＂W＂状。生长素受体DL-TIR1和＂可能的＂生长素受体DL-ABP1在龙眼体胚中的变化趋势极为相似。

英文摘要：

The RT-PCR with RACE method was used to clone the complete mRNA sequences of the transport inhibitor response 1 gene（DL-TIR1）and auxin binding protein 1 gene（DL-ABP1）from embryogenic calli of longan（Dimocarpus longan Lour.）.And bioinformatics methods were used to analyze obtained sequences and putative amino acid sequences.Then qRT-PCR（real-time reverse transcription PCR）method was used to determine the mRNA transcription level of two genes in the process of somatic embryogenesis in longan.The results were as follows：The full length TIR1（DL-TIR1,GenBank,GQ870264）mRNA,about 2 328 bp,consisting of an open reading frame of 1 761 bp,and 5＇and 3＇untranslated regions of 118 bp and 449 bp,respectively.The putative protein had 586 amino acids,and the identity with the other polypeptides varied between 85%-57%.DL-TIR1 expressed in 8 different stages during somatic embryogenesis,which showed approximately a＂W＂curve.The full length ABP1（DL-ABP1,GenBank,GQ900592）mRNA,about 869 bp,consisting of an open reading frame of 567 bp,and 5＇and 3＇untranslated regions of 43 bp and 259 bp,respectively.The putative protein had 188 amino acids,and the identity with the other polypeptides varied between 87%-72%.The expression of DL-ABP1 in 8 different stages during somatic embryogenesis also showed approximately a＂W＂curve.The mRNA transcription level of the auxin receptor gene DL-TIR1 and the potential auxin receptor gene DL-ABP1 were very similar.