中文摘要：

利用聚合酶链式反应（PCR）克隆出集胞藻Synechocystis sp.PCC 6803变藻蓝蛋白和藻蓝蛋白α亚基的编码基因apcA和cpcA,将脱辅基蛋白ApcA、CpcA分别与裂合酶CpeS1或CpcE/F以及血红素氧化酶HO1、胆绿素还原酶PcyA在大肠杆菌中共同表达。研究表明：通过大肠杆菌体内重组可以获得具有光学活性的重组色素蛋白PCB-ApcA和PCB-CpcA。吸收光谱、荧光光谱以及锌电泳均表明,藻蓝胆素与脱辅基蛋白形成了正确的共价连接。由此可知,利用大肠杆菌进行集胞藻藻蓝蛋白和变藻蓝蛋白α亚基的体内生物合成是可实现的。同时还对重组色素蛋白的摩尔消光系数和荧光量子产率进行了计算。

英文摘要：

Genes of α-subunit of allophycocyanin and phycocyanin（apcA and cpcA） from Synechocystis sp.PCC 6803 were cloned by PCR and expressed in E.coli.ApcA and CpcA were coexpressed in E.coli with heme oxygenase and 3Z-phycocyanobilin：ferredoxin oxidoreductase（HO1 and PcyA） and lyase（CpeS1 or CpcE/F） through in vivo reconstitution tests.All results from SDS-PAGE of Zn2＋-induced fluorescence,absorption and fluorescence spectra showed that ApcA and CpcA could bind covalently phycocyanobilin（PCB） in E.coli and in vivo biosynthesis of α-subunit of phycocyanin and allophycocyanin from Synechocystis sp.PCC 6803 was practicable.Molar extinction coefficient and fluorescence quantum yield of reconstitution chromoproteins were calculated in the paper.