【目的】建立山楂叶螨(Tetranychus vienensis Zacher)室内饲养方法并研究其种群生命表,为山楂叶螨的室内研究与综合治理奠定基础。【方法】在温度(25±1)℃、相对湿度(60±10)%和光周期16L∶8D条件下,研究山楂叶螨火棘离体叶片单头饲养法、火棘植株饲养法和火棘枝条水培饲养法3种饲养方法,并采用火棘离体叶片单头饲养法组建山楂叶螨自然种群和室内继代饲养种群的生命表。【结果】在室内条件下,3种方法均可以连续饲养山楂叶螨;室内继代饲养种群幼螨和前若螨的发育历期、成螨产卵前期和雌螨寿命均与自然种群差异不显著,但室内继代饲养种群的卵和后若螨的发育历期均显著短于自然种群,室内继代饲种群雌雄比例和单雌日均产卵量均高于自然种群。山楂叶螨室内继代饲养种群的净值增长率R0(9.113)、内禀增长率rm(0.096)和周限增长率λ(1.101)均高于自然种群的相应参数,而山楂叶螨室内继代饲养种群的平均世代周期(22.780d)低于自然种群(24.355d)。【结论】建立了以火棘为寄主植物室内饲养山楂叶螨的方法;在实验室条件下,室内继代饲养的山楂叶螨种群比新采集的田间自然种群具有更大的种群增长潜力。
[Objective] This study established rearing methods for Tetranychus vienensis Zacher in la- boratory and researched the life-table to provide basis for its integrated management. [Method] Three methods using detached Pyracantha fortuneana leaves, water-cultured P. fortuneana branches and soilcultured P. fortuneana plants were adapted to rear T. vienensis with the host plant of P. fortuneana under condition of temperature (25±1) ℃ ,humidity (60±10)% ,and photoperiod 16 :8 (L : D) in laboratory. The life-table of T. vienensis laboratory population and natural population was also established using the detached leaves method. [Result] Tetranychus vienensis were reared in laboratory using all the three methods. There was no significant difference in developmental duration of larva and protonymph stage,the preoviposition period and female longevity between the laboratory and natural populations, but the developmental duration of egg and deutonymph stage in laboratory was significantly shorter than natural population. The sex ratio (female:male) and average egg per female in laboratory were higher than those of natural population. The life table showed that net reproduction rate R0 (9. 113), intrinsic increasing rate rm (0. 096) and finite increasing rate λ (1. 101) of laboratory population were higher than those of the natural population. The mean generation time T (22. 780 d) of laboratory population was lower than that of the natural population (24. 355 d). [Conclusion] Three laboratory rearing methods for T. vienensis were set up and the laboratory population showed higher population increasing potentiality than natural population.