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中文摘要：
以江西铅山红芽芋（Colocasia esculenta L.Schott var.cormosus‘Hongyayu’）试管苗为材料,建立了芋球茎片两步法离体快繁体系,并对其再生苗的形态指标、染色体数目、生理和光合特性以及叶绿素荧光特性进行了检测。结果表明：（1）红芽芋球茎片单芽诱导的最佳培养基为MS＋KT 2 mg/L＋6-BA 1 mg/L＋NAA0.1mg/L,诱导培养30d后将单芽从球茎片上分离,再接种到生根培养基（MS＋KT 2mg/L＋NAA 0.1mg/L）上培养30d即可形成完整植株,移栽成活率高达98%;（2）由球茎片单芽、丛生芽、不定芽离体快繁获得的红芽芋再生苗在形态指标、叶下表皮气孔参数、染色体数目、生理生化指标以及叶片光合特性参数和叶绿素荧光特性方面均无显著差异。说明红芽芋球茎片两步法离体培养的再生苗繁殖系数高、染色体数目稳定,该离体快繁体系可应用于江西铅山红芽芋的工厂化生产。
英文摘要：
Using Jiangxi Yanshan red bud taro（Colocasia esculenta L.Schott var.cormosus‘Hongyayu＇）plantlets as materials,an in vitrotwo-step micro-propagation system for red bud taro corm segments was studied in this paper, and the morphology, cytology, physiology,photosynthetic characteristics and chlorophyll fluorescence characteristics of its plantlets were also observed and detected.Results showed that：（1）The best single bud induction medium for red bud taro corm segments was MS ＋ KT 2 mg/L ＋ 6-BA 1 mg/L ＋ NAA 0.1 mg/L.After 30 days,single buds induced from corm segments were separated and transferred onto the rooting medium（MS ＋ KT 2 mg/L＋ NAA0.1 mg/L）and were able to grow into complete plants.After 30 days,red bud taro plantlets were removed and transplanted,and the survival rate reached more than98%.（2）There were no significant differences in morphological indexes,photosyntheticcharacteristics,leaf epidermis stomatal parameters,chromosome numbers,physiological and biochemical indexes,photosynthetic characteristics parameters and chlorophyll fluorescence characteristics among regenerated plantlets from single bud corm segments,regenerated plantlets from multiple buds or plantlets regenerated from adventitious buds.Therefore,the two-step micropropagation in vitro system for red bud taro corm segments was able to ensure genetic stability,and provides a theoretical reference for Jiangxi Yanshan red bud taro factory production.
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