中文摘要：

利用中国原子能科学研究院600kV高压倍加器产生的14MeV的单能中子，以不同剂量对不含和含有甘露醇或香兰素衍生物VND3207的质粒DNA进行了照射，使用凝胶电泳技术观测了DNA分子的链断裂。结果表明，中子可诱发DNA分子发生单链和双链断裂，随剂量的升高，链断裂产额逐渐增加，具有明显的剂量响应。在含有甘露醇和VND3207的研究体系中，也存在一定的剂量响应，但相比不含两者的体系而言，剂量响应不明显，且在相同剂量下DNA分子的链断裂产额极低。此结果反映出研究中所选用浓度的甘露醇与VND3207可有效降低中子辐射所诱发的DNA单链和双链断裂的产生，对DNA分子起到极好的保护作用，具有一定的中子辐射防护能力。

英文摘要：

Using neutrons of 14 MeV generated by 600 kV Cockcroft-Walton accelera- tor, the DNA samples with or without mannitol or VND3207 were irradiated with various absorbed doses, and the DNA strand breaks were determined by means of gel electrophoresis. The results indicate that neutron can produce the DNA single- and double-strand breaks, and the yields of strand breaks increase with the dose increment, i.e. there is a distinct dose response. Although for the solution system with additive of mannitol or VND3207, there is also a dose response, the yields of DNA strand breaks are much less than that without mannitol or VND3207 solution. It can be suggested that mannitol or VND3207 reduces the yields of DNA single- and double-strand breaksinduced by neutron irradiation efficiently, and mannitol or VND3207 has stronger capa- bility ofradioprotection against neutron radiation.