中文摘要：

目的介绍一种新的简便有效的体外培养和纯化雪旺细胞（SCs）方法，以获取足够数量、高纯度的SCs。方法取新生3～5d的SD乳鼠坐骨神经和臂丛神经，用胶原酶消化后收集细胞进行原代培养，细胞接种浓度为3×10^5个／mL，在接种72h内用胰酶分步多次消化进行提纯。结果该方法与1．25g／L胰酶差速酶消提纯SCs相比．能更有效地将SCs和成纤维细胞分离，经免疫荧光染色显示，SCs的纯度能达到95％以上。结论本方法简便易掌握，可重复性高，能获得足够数量、高纯度的SCs供细胞移植研究。

英文摘要：

Objective To describe a simple and efficient method to obtain large quantities of highly purified Schwann cells （SCs）. Methods SCs were isolated from the sciatic and brachial nerves of 3- to 5-day-old newborn SD rats by collagenase digestion. The isolated SCs were plated at the density of 3×10^5/mL for primary culture, several rounds of trypsin digestion were performed within 72 h to purify SCs. Results Compared with the purification using 1.25 g/L trypsin digestion in serial differential detachment procedures, our protocol allowed easier and more efficient separation of the SCs from the fibroblasts. Immunocytochemical staining showed that the purity of the SCs exceeded 95%. Conclusion The purification protocol of the SCs we established can be easily carried out and yields well reproducible results to obtain large quantities of highly purified SCs for transplantation studies.