中文摘要：

目的探讨姜黄素联合细胞因子活化的杀伤细胞（CIK cells）杀伤肾癌细胞逆转耐药P-糖蛋白（P-gp）表达的作用。方法培养耐阿霉素的肾癌细胞系（786-O/ADR）,观察不同浓度姜黄素和CIK细胞单独或联合处理后,应用cck-8检测细胞活性,免疫荧光检测P-gp的表达变化,TUNEL和caspase-3染色观察细胞凋亡情况。结果姜黄素（浓度分别为20μM和40μM）、CIK细胞（分别为20：1和40：1）单独应用以及姜黄素＋CIK细胞联合应用组均能显著降低P-gp的表达水平,降低细胞活性,下调caspase-3表达水平（〈0.05）。结论姜黄素联合CIK细胞逆转肾癌细胞耐药蛋白P-gp表达,促进细胞凋亡,发挥协同作用。

英文摘要：

Objective To investigate the effect of combination of curcumin and cytokine-induced killer（CIK） cells on the expression of P-glucose protein in renal carcinoma786-O/ADR cells. Methods The renal carcinoma cell Doxorubicin-resistance cell line（786-O/ADR） was cultured and treated by different concentrations of curcumin and CIK cells individually or in combination. The cell viability was observed by CCK-8 kit, the expression of P-gp was observed by immunofluorescence, and the apoptosis was observed by caspase-3 immunostaining and TUNEL assay. Results The expression levels of P-gp in 786-O/ADR cells and the cell viability were decreased significantly（ 〈0.05）, and the expression level of caspase-3 was downregulated after treatment of curcumin（20μM and 40μM） or CIK cells（20 ： 1 and 40 ： 1） or the combination of curcumin＋ CIK cells. Conclusion The combination of curcumin and CIK cells reverses the expression of P-glucose protein in 786-O/ADR cells, would be used in treatment of renal carcinoma.