中文摘要：

目的：建立牛至中原儿茶酸的含量测定方法，并测定不同产地牛至药材中原儿茶酸的含量。方法：采用高效液相色谱法，Attima C18柱（4．6mm×250mm，5μm），流动相为甲醇-0.15％醋酸水溶液（9：91），流速1．0mL·min^-1，检测波长260nm。结果：线性回归方程为Y=-28．83＋3267X，r=0．9999．线性范围为0．0966～0．4832μg，平均回收率为99．66%。10个不同产地牛至药材，原儿茶酸含量存在一定差异。结论：本方法简便、稳定、可靠，可用于牛至中原儿茶酸的含量测定。不同产地牛至药材中原儿茶酸的含量存在一定差异．以贵州、安徽产牛至药材中原儿茶酸的含量较高。

英文摘要：

OBJECTIVE To establish a method for determining the content of protocatechuic acid in Origanum vulgate, and to determine protocatechuic acid in Origanurn vulgare from different production place. METHODS The chromatographic conditions were Attima C18 column, mobile phase of Methanol -0. 15% acetic acid （9：91）. Flow rate was 1.0 mL·min^-1. Detection wavelength was at 260 nm. RESULTS The regression equation was Y = - 28.83 ＋ 3 267X （r = 0. 999 9） and the linear range was 0. 096 6～0. 483 2 μg. The average recovery was 99. 96%. The contents of protocatechuic acid in Origanum vulgate from ten different places of origin was different. CONCLUSION The method is simple, stable and reliable. It can be used for content determination of protocatechuic acid in Origanurn vulgate. The content of protocatechuic acid in Origanum vulgate from Guizhou and Anhui are high.