中文摘要：

以番茄果实为材料,采用颈环引物的方法对番茄果实中的miRNA159和miRNA168进行反转录,并对得到的目的片段进行扩增,通过琼脂糖凝胶电泳观察得到相应大小的目的片段。对miRNA159所得大小正确的片段进行测序,验证所得片段为正确的目的片段。这种低成本的miRNA检测方法简单、实用,适用于生物信息预测及深度测序得到的低拷贝的新miRNA的快速验证。

英文摘要：

The paper used tomato as material,and a stem-loop sequence as the reverse transcription primer to amplify miRNA159 and miRNA168.Analyzed the bands which was the right size,made sure that it＇s the right bands we wanted.So this method which cost less and operate simply ould make a great help for the research of the characters and functions of miRNA.