中文摘要：

全内反射荧光显微术（total internal reflection fluorescence microscopy，TIRFM）是一种灵敏、快速的单分子成像和检测技术，近年来得到迅猛发展。该技术已广泛应用于生命科学、化学、物理学等领域。本文综述了全内反射荧光显微术的原理及其在活细胞单分子检测中的应用，并对其发展前景进行了展望。

英文摘要：

Total internal reflection fluorescence microscopy （TIRFM） is a sensitive and fast singlemolecule detection and imaging technique. It has been successfully applied to the fields of life science, chemistry and physics, particularly the area of single-molecule detection in living ceils. In this paper, we reviewed the principle of TIRFM and the applications of TIRFM for single-molecule detection in living cells. We also prospected the development of TIRFM.