中文摘要：

目的了解精子蛋白Sp32／OY—TES-1基因mRNA的表达特点，研制癌-睾丸抗原（CTA）的肿瘤疫苗。方法采用逆转录-聚合酶链反应（RT-PCR）技术，检测95例正常人组织及125例人肿瘤组织中Sp32／OY—TES-1 mRNA的表达，随机选取RT-PCR阳性产物12份进行DNA测序；对所得数据进行统计学分析。结果Spa2／OY—TES-1在正常和肿瘤组织中的表达频率分别为68．4％（65／95）和44．00％（55／125），两者比较有统计学意义（P〈0．05）。在24种不同器官的正常组织中，21种表达Sp32／OY—TES—1基因mRNA（87．50％）。DNA测序结果证实，RT-PCR产物为Sp32／OY—TES-1基因。结论Sp32／OY—TES-1基因的mRNA在正常组织中广泛表达，它是否可归为睾丸和肿瘤限制性表达的基因尚需进一步研究。

英文摘要：

Objective To investigate the mRNA expressive profile of Sp32/OY-TES-1 gene. Methods Ninety-five spcimens of human normal tissues and 125 specimens of human tumor tissues were analyzed for the expression of OY-TES-1 by reverse transcription polymerase chain reaction （RT-PCR）. Twelve RT-PCR positive products were randomly selected and sequenced. The data obtained were staticly analized. Results The expressive frequencies of Sp32/OY-TES-1 in normal and tumor tissues were 68.42 % （65/95） and 44.00 % （55/125 ）, respectively, which were of significant difference （ P 〈 0.05 ）. On 24 specimens of normal tissues from different organs, 21 were positive in Sp32/OY-TES-1 （87.50 % ）. DNA sequencing results further confirmed that RT-PCR products were truly Sp32/OY-TES-1 eDNA. Conclusion With ubiquitous expression in human normal tissues, whether Sp32/OY-TES-1 is a gene expressed strictly in testis and tumor tissues or not needs to be further determined.