中文摘要：

目的探讨补肾活血饮治疗帕金森病（PD）的作用机制。方法应用脑右侧黑质中注射6-羟基多巴胺（6-OHDA）的方法制备大鼠偏侧PD模型。将120只SD大鼠随机分为正常对照组（n=20），模型组（n=58），补肾活血饮治疗组（n=42）：每日灌胃补肾活血饮，按成人（60kg）每公斤体重剂量10倍计算。治疗8周后断头处死取脑，采用逆转录-聚合酶链反应（RT-PCR）测定大鼠脑黑质孤儿核受体（Nurrl）的mRNA表达；免疫组化检测大鼠黑质内酪氨酸羟化酶（TH）阳性细胞计数。结果与正常对照组比较，模型组脑黑质Nurrl mRNA表达明显下降（0.22±0.03比1.10±0.27，P〈0.01），TH阳性细胞计数明显减少[（5.4±2.6）个比（104.3±26.4）个，P〈0.01]。补肾活血饮组脑黑质Nurrl mRNA表达（0.97±0.15）较模型组增高（P〈0.01），与正常对照组比较差异无统计学意义；黑质内可见大量TH免疫阳性细胞[（49.4±14.7）个]，显著多于模型组，但较正常对照组显著减少（P均〈0.01）。结论补肾活血饮可能通过增加PD模型大鼠脑组织内Nurrl及TH含量起到治疗PD的作用。

英文摘要：

Objective To approach the therapeutic effect and mechanism of Bushen Huoxue Yin（补肾活血饮） in treatment of Parkinson disease （PD）. Methods Unilateral PD model was made by injecting 6-hydroxydopamine （6-OHDA） stereotaxically into right side of the mesencephalic ventral tegmental area. A normal control group （n=20）, a normal saline control group or a model group （n=58） and a Bushen Huoxue Yin （a decoction） treatment group （n=42） were set up randomly. In the Bushen Huoxue Yin treatment group, the decoction was infused to the stomach everyday, the dosage was calculated in accord to 10 times of that of an adult （60 kg）, and after 8 weeks of treatment, the rat head was cut and its brain was taken out. The expression of retinoid-related nuclear orphan receptor 1 （Nurrl） mRNA was detected by reverse transcription-polymerase chain reaction （RT-PCR） in each group; the number of tyrosine hydroxylase （TH） positive cells in substantia nigra of rats in each group were measured by immunohistochemical methods. Results Compared with the normal control group, the expression of Nurrl mRNA in the rat brain of the model group was decreased significantly （0.22±0.03 vs. 1.10±0.27, P〈0.01）, and the TH positive cells were reduced in number [（5.4±2.6） cells vs.（104.3±26.4） ceils, P〈0.01]; in the Bushen Huoxue Yin treatment group, the expression of Nurrl mRNA in the rat brain （0.97±0.15） was higher than that of the model group （P〈0.01）, and compared with the normal control group, there was no statistical significance; in the substantia nigra, there were a lot of TH immune positive cells [（49.4±14.7） cells], markedly higher than those in the model group, but obviously less than that in the normal control group （both P〈0.01）. Conclusion The mechanisms of Bushen Huoxue Yin in treatment of PD are possibly due to its effects of increasing the contents of Nurrl and TH in the PD rat brain.