中文摘要：

与传统微生物生态学分析方法相比,近几年高通量测序以速度快、通量高、低成本等特征在微生物多样性检测方面得到了充分应用,并采用此方法研究温度对微生物浸出赞比亚一种低品位铜矿的效率和群落生态的影响。结果表明：在30℃和45℃浸出条件下,铜浸出率分别为64.2%和69.4%,45℃条件下获得了较高铜浸出率。采用高通量Illumina Miseq测序技术分析30℃和45℃浸出条件下微生物群落结构及其动态变化,当浸出温度为30℃,Acidithiobacillus caldus和Acidiphilium sp.是主要浸矿菌;当浸出温度为45℃时,Sulfobacillus sp.和Acidithiobacillus caldus是优势种群。

英文摘要：

The main features of high-throughput sequencing are high speed, high-throughput and low cost compared with the traditional microbial ecology analysis method. In recent years, the high-throughput sequencing technology gets full uses in the investigation of mierobial diversity. The effects of temperature on bioleaching of low grade copper ore and the dynamics of microbial eommunity from Zambia were studied. The results show that, at 30 ℃and 45 ℃, the copper leaching rates are 64,2% and 69.4%, respectively. The rise of temperature results in higher copper leaching rate at 45 ℃. The shift of microbial community at 30 ℃ and 45 ℃ during bioleaching process was analyzed by Illumina Miseq sequencing platform. The results show that Acidithiobacillus caldus and Acidiphilium sp. are the main microorganisms at 30 ℃. While at 45 ℃, Sulfobacillus sp. and Acidithiobacillus caldus dominate in the microbial community.