中文摘要：

许多植物激素通过 modulating 蛋白质降级调整植物生长和开发。在这研究， proteome 研究基于多维的非胶化猎枪途径被执行经由 Arabidopsis thaliana 的 ubiquitin-proteasome 小径分析导致植物生长素的蛋白质降级，与学习在植物生长素处理以后的全面蛋白质变化的强调(1 nM 或 1 湯愠楢楬祴。

英文摘要：

Many phytohormones regulate plant growth and development through modulating protein degradation. In this study, a proteome study based on multidimensional non-gel shotgun approach was performed to analyze the auxin-induced protein degradation via ubiquitinproteasome pathway of Arabidopsis thaliana, with the emphasis to study the overall protein changes after auxin treatment （1 nM or 1 μM indole-3-acetic acid for 6, 12, or 24 h）. More than a thousand proteins were detected by using label-free shotgun method, and 386 increased proteins and 370 decreased ones were identified after indole-3-acetic acid treatment. By using the auxin recep- tor-deficient mutant, tirl-1, as control, comparative analysis revealed that 69 and 79 proteins were significantly decreased and increased, respectively. Detailed analysis showed that among the altered proteins, some were previously reported to be associated with auxin regulation and others are potentially involved in mediating the auxin effects on specific cellular and physiological processes by regulating photosynthesis, chloroplast development, cyto-skeleton, and intracellular signaling. Our results demonstrated that label-free shotgun proteomics is a powerful tool for large-scale protein identification and the analysis of the proteomic profiling of auxin-regulated biological processes will provide informative clues of underlying mechanisms of auxin effects. These results will help to expand the understanding of how auxin regulates plant growth and development via protein degradation.