中文摘要：

瞄准：观察胰腺、十二指肠的 homeobox factor-1 是否提高胰腺的管的上皮细胞的区别进生产胰岛素的房间在试管内。方法：老鼠胰腺的织物被 collegenase 提交到消化，管的上皮细胞被密度坡度远心沉淀分开然后在与 10% 胎儿的牛的浆液中等的 RPMI1640 有教养。在 3-5 段落以后，房间在再结合原生质标志 XlHbox8VP16 的 transfection 前为 24 h 在一个六井的盘子中被孵化。Lightcycler 量的即时 RT-PCR 被用来在胰腺的上皮细胞检测 PDX-1 和胰岛素 mRNA 的表示。PDX-1 和胰岛素蛋白质的表示被西方的弄污分析。胰岛素分泌物被放射性免疫测定检测。生产胰岛素的房间被染色 dithizone 检测。结果：XlHbox8 mRNA 在胰腺的管的上皮细胞被表示。象 PDX-1 和胰岛素蛋白质一样的 PDX-1 和胰岛素 mRNA 显著地在 transfected 组被增加。区分开来与胰腺的管的上皮细胞的生产胰岛素的房间的生产和胰岛素分泌物比有有效差量的 untransfected 房间在试管内的那些高(1.32 +/- 0.43 对 3.48 +/- 0.81， P 【 0.01 在 5.6 mmol/L；4.86 +/- 1.15 对 10.25 +/- 1.32， P 【 0.01 在 16.7 mmol/L ) 。结论：PDX-1 罐头 differentiate 老鼠胰腺的管的上皮细胞进生产胰岛素的房间在试管内。在试管内 PDX-1 transfection 是为增加生产胰岛素的房间的来源的珍贵策略。

英文摘要：

AIM： To observe whether pancreatic and duodenal homeobox factor-1 enhances the differentiation of pancreatic ductal epithelial cells into insulin-producing cells in vitro. METHODS： Rat pancreatic tissue was submitted to digestion by collegenase, ductal epithelial cells were separated by density gradient centrifugation and then cultured in RPMI1640 medium with 10% fetal bovine serum. After 3-5 passages, the cells were incubated in a six-well plate for 24 h before transfection of recombination plasmid XlHbox8VP16. Lightcycler quantitative real-time RT-PCR was used to detect the expression of PDX-1 and insulin mRNA in pancreatic epithelial cells. The expression of PDX-1 and insulin protein was analyzed by Western blotting. Insulin secretion was detected by radioimmunoassay. Insulin- producing cells were detected by dithizone-staining. RESULTS： XlHbox8 mRNA was expressed in pancreatic ductal epithelial cells. PDX-1 and insulin mRNA as well as PDX-1 and insulin protein were significantly increased in the transfected group. The production and insulin secretion of insulin-producing cells differentiated from pancreatic ductal epithelial cells were higher than those of the untransfected cells in vitro with a significant difference （1.32 ± 0.43 vs 3.48 ± 0.81, P 〈 0.01 at 5.6 mmol/L; 4.86 ± 1.15 vs 10.25 ± 1.32, P 〈 0.01 at 16.7 mmol/L）. CONCLUSION： PDX-1 can differentiate rat pancreatic ductal epithelial cells into insulin-producing cells in vitro. In vitro PDX-1 transfection is a valuable strategy for increasing the source of insulin-producing cells.