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Molecular Cloning and cDNA Sequence Analysis of Two New Lepidopteran OR83b Orthologue Chemoreceptors
  • 时间:0
  • 分类:Q78[生物学—分子生物学] Q959.223.5[生物学—动物学]
  • 作者机构:[1]Agro-Environmental Protection Institute, Ministry of Agriculture/Farmland Ecosystem Observation and Experiment Station of Genetically Modified Organisms in Wuqing, Chinese Academy of Agricultural Sciences, Tianjin 300191, P.R.China, [2]Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture/Entomology Department, Plant Protection College, Nanjing Agricultural University, Nanjing 210095, P.R. China, [3]Plant Protection College, Shandong Agricultural University, Tai 'an 271018, P.R. China
  • 相关基金:Acknowledgements This work was supported by funds from the National Natural Science Foundation of China (30800725 and 30770278) and the Central Public Research Institutes Basic Funds for Research and Development (Agro-Environmental Protection Institute, Ministry of Agriculture, China).
  • 相关项目:甜菜夜蛾触角中性信息素降解酯酶的分子克隆和功能表达
中文摘要:

这研究的目的是孤立全身的 cDNA 序列一高度不平常地从 Spodoptera exigua 和 S 的天线保存了嗅觉的受体(到果蝇 melanogaster DOR83b 的 orthologue ) 。由使用反向的抄写聚合酶链反应(RT-PCR ) 和 cDNA 的快速的扩大的 litura 结束(种族) 方法。生物信息学方法被用来进一步分析 cDNA 序列和通常认为的氨基酸序列。从嗅觉的受体的二个全身的 cDNA 序列(或) 男 S。exigua 和 S。litura 分别地作为 SexiOR2 和 SlitOR2 被说出。分别地, SexiOR2 和 SlitOR2 与 473 残余的推出的氨基酸序列由 1 906 和 2 483 bp 的核苷酸顺序组成了。顺序分析显示 cDNA 的推出的氨基酸序列从以前报导的蛾与 OR83b orthologue 化学受体序列分享了高相同,暗示 cDNA 序列具有 OR83b orthologue 化学受体基因。

英文摘要:

The aim of this study was to isolate the full-length cDNA sequences of an unusually highly conserved olfactory receptor (orthologue to the Drosophila melanogaster DOR83b) from the antennae of Spodoptera exigua and S. litura by using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Bioinformatics methods were used to further analyze the cDNA sequences and putative amino acid sequences. The two full-length cDNA sequences from olfactory receptor (OR) of male S. exigua and S. litura were named as SexiOR2 and SlitOR2, respectively. SexiOR2 and SlitOR2 consisted of nucleotide sequence of 1 906 and 2 483 bp, respectively, and both with deduced amino acid sequences of 473 residues. The sequence analysis indicated that the deduced amino acid sequences of the cDNA shared the high homologies with OR83b orthologue chemoreceptor sequences from previously reported moths, implying that the cDNA sequences were of OR83b orthologue chemoreceptor genes.

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