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中文摘要：
目的：探讨转染Elafin对百草枯（Paraquat,PQ）诱导的肺泡上皮凋亡的影响及可能机制。方法：电穿孔法转染p EGFP-C1-Elafin进入A549细胞,培养24 h后,RT-PCR检测Elafin mRNA在A549细胞中的表达情况;并给予生理盐水、不同浓度PQ（5、50、100μmol/L）作用后,Annexin V/PI双染法流式细胞仪检测A549细胞凋亡率、活性氧类物质（Reactive oxygen species,ROS）含量。Western blot检测核因子相关因子2（Nuclear factor erythroid like-2,Nrf2）、血红素加氧酶-1（Heme oxygenase-1,HO-1）表达情况。结果：转入A549细胞Elafin成功表达。PQ呈浓度依赖性导致A549细胞凋亡;PQ可使ROS含量明显增加,Nrf2、HO-1表达下调。Elafin可抑制PQ诱导的细胞调亡,上调Nrf2、HO-1蛋白表达。结论：Elafin可以一定程度抑制PQ诱导的A549细胞凋亡,其机制可能与其上调抗氧化蛋白Nrf2等有关。
英文摘要：
Objective： To investigate the effect of recombinant Elafin on A549 apoptosis induced by paraquat and the underlying mechanism. Methods： p EGFP-C1-Elafin was transformed into A549 competent cells by electroporation. Transformed A549 was cultured for additional 24 h before Elafin mRNA was detected by RT-PCR and cocultured with or without various concentration of paraquat（ PQ） for different duration. A549 apoptosis percentage and reactive oxygen species（ ROS） content were tested by flow cytometry. Nuclear factor erythroid like-2（ Nrf2） heme oxygenase-1（ HO-1） were examed by Western blot. Results： Elafin expressed successfully in A549 after transformation. PQ caused A549 apoptosis in a concentration dependent manner and leaded to ROS content increasing and Nrf2 and HO-1 decreasing. However,elafin could inhibit ROS production and A549 apoptosis,upregulate Nrf2 and HO-1. Conclusion： Elafin could restrict A549 apoptosis to some extent and the possible mechanism lied in its ability to upregulate Nrf2 expression.
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