中文摘要：

目的观察黄芪、苏木及其组方对荷瘤小鼠脾CD4＋CD25＋Foxp3＋调节性T细胞表达及血清细胞因子水平的干预作用。方法复制C57BL/6小鼠Lewis肺癌动物模型,动态观察黄芪、苏木及其组方对抑瘤率、肺转移抑制率、荷瘤小鼠体重、生存期的影响。接种后6、10、15、20d流式细胞仪检测荷瘤小鼠脾CD4＋CD25＋Foxp3＋调节性T细胞的表达,20d采集小鼠血清,流式微球阵列技术检测细胞因子IL-2、IL-6、IL-10、IL-12p70、IFN-γ水平,酶联免疫法（ELISA）法检测转化生长因子β（TGF-β）水平。结果苏木组6d、黄芪＋苏木组10d瘤重低于荷瘤对照组（P〈0.05）;黄芪＋苏木组肺转移抑制率为77.75%;荷瘤对照组小鼠脾CD4＋CD25＋Foxp3＋细胞表达随荷瘤天数的增加呈上升趋势,20d黄芪组小鼠脾CD4＋CD25＋Foxp3＋细胞表达低于荷瘤对照组（P〈0.05）,黄芪＋苏木组显著低于荷瘤对照组（P〈0.01）;黄芪＋苏木组小鼠血清IL-10、TGF-β水平低于荷瘤对照组（P〈0.05）;中位生存期分别为黄芪＋苏木组（39d）〉黄芪组（38d）〉苏木组（35d）〉荷瘤对照组（24d）。结论益气活血中药组方黄芪＋苏木可通过调控荷瘤小鼠脾细胞中调节性T细胞表达改善其体内存在的免疫耐受状态,其作用优于单一益气药或活血药,这可能是中药抑制小鼠Lewis肺癌生长转移、延长生存的作用靶点之一。

英文摘要：

Objective To observe the interventional effects of Huangqi （Radix Astragali） and Sumu （Lignum Sappan） and their formulas on expression of spleen CD4＋CD25＋Foxp3＋ regulatory T cells （Treg） and serum level of cytokines in tumor-bearing mice.Methods The model of Lewis pulmonary cancer was established in C57BL/6 mice.The influences of Huangqi,Sumu and their formulas on the tumor inhibitory rate,inhibitory rate of lung metastasis,weight and survival time were observed dynamically in tumor-bearing mice.The expression of CD4＋CD25＋Foxp3＋ Treg detected by using flow cytometer 6,10,15 and 20 days after inoculation respectively.The levels of IL-2,IL-6,IL-10,IL-12p70 and IFN-γ were detected by using CBA FLEX SET and the level of TGF-β was detected by using ELISA.Results The tumor weight was lower in 6-day Sumu group and 10-day Huangqi＋Sumu group than that in the control group （P0.05）.The inhibitory rate of lung metastasis was 77.75% in Huangqi＋Sumu group.The expression of spleen CD4＋CD25＋Foxp3＋ Treg trended to ascend along with the increase of tumor-bearing day in the control group,and that was lower in 20-day Huangqi group （P0.05） and significantly lower in Huangqi＋Sumu group （P0.01） than that in the control group.The serum levels of IL-10 and TGF-β were lower in Huangqi＋Sumu group than those in the control group （P0.05）.The descending order of median survival time showed as follows：Huangqi＋Sumu group （39 days）,Huangqi group （38 days）,Sumu group （35 days） and control group （24 days）.Conclusion The compound formula （Huangqi＋Sumu） with the actions of supplementing qi and activating blood circulation can improve the immune tolerance condition through regulating the expression of spleen Treg in tumor-bearing mice,with the better effect than single Huangqi or Sumu.It may be one of effective targets of Chinese herbal remedies for inhibiting Lewis lung metastasis and prolonging life.