中文摘要：

以2种独行菜种子为试材,采用cDNA-SRAP技术,研究了dNTPs、Mg2＋、上下游引物、cDNA模板、Taq DNA聚合酶浓度等5个因素对独行菜cDNA-SRAP反应体系扩增结果的影响,以期得到适合2种独行菜种子的cDNA-SRAP反应体系,并且对cDNA-SRAP扩增条件进行了优化。结果表明：优化的最佳cDNA-SRAP反应体系（20μL）为10×PCR buffer,cDNA模板用量100ng,Mg2＋浓度1.75mmol·L-1,dNTPs浓度0.275mmol·L-1,引物浓度1.0μmol·L-1,Taq DNA聚合酶用量0.5U;利用优化的cDNA-SRAP反应体系能够扩增获得清晰、数目多的条带,可用于后续深入研究2种独行菜不同处理下差异表达基因。

英文摘要：

A cDNA sequence-related amplified polymorphism（SRAP）reaction system of two species Lepidium L.were established and optimiazed by orthogonal experimental design and single factor analysis of different concentration of dNTPs,Mg2＋,primer,Taq DNA polymerase and DNA template was conducted.The results showed that the optimal reaction system for the two species of Lepidium L.was followed as 0.275 mmol·L-1 dNTPs,0.5 U Taq DNA polymerase,1.0μmol·L-1 primer,100 ng template cDNA in total 20μL of reaction solution.Using the optimal system of cDNA-SRAP,clear stable and repeatable bands could be amplified from two species of Lepidiumplants for analyzing gene differential expression.