中文摘要：

本研究采用基于PCR技术的siRNA表达方法，快速筛选到两个可以抑制HBV基因表达的siRNAs序列；S2和X1.S2和X1被克隆到人泡沫逆转录病毒（human foamy virus，HFV）载体中，构建成为分别表达S2和X1的单表达载体和同时表达S2和X1的双表达载体。将siRNAs表达载体转导入HepAD38细胞系中，多种检测分析的结果表明这些siRNA表达载体可以有效抑制多个HBV基因的表达和病毒DNA复制；并且其抑制作用是长期的，可以持续到转导后3个月。

英文摘要：

A polymerase chain reaction （PCR）-based siRNA expression strategy was employed to rapidly screen for effective siRNA sequences. Two effective siRNAs sequences （designated as S2 and X1） which reduced the HBV RNA by〉90% were identified. For delivering the siRNAs, they were cloned into a human foamy virus （HFV） based vector to generate single siRNA expression vectors HFVU6-siS2, HFVU6-siX1 and a dual siRNA expression vector HFVU6-siSX. After indrodcing the siRNAs into HepAD38 cell lines, the expression level of HBV genes and the replications of HBV were analyzed by ELISA, Western blot, Quantitative PCR analysis, RT-PCR and Southern blot. It was identified that these siRNA vectors can effectively inhibit multiple HBV gene expression and viral DNA replication. In addition, the repression of HBV RNA and DNA was stable up to 3 months posttransduction.